大口黑鲈北方亚种和佛罗里达亚种的同工酶分析

Journal of Tianjin Agricultural University ›› 2012, Vol. 19 ›› Issue (3): 8-12.

• Researches and Scientific Notes • Previous Articles Next Articles

Isozyme Analysis of Northern and Florida Subspecies of Largemouth Bass Micropterus salmoides

ZHANG Da-li¹, YANG Qiang¹, HAO Jun¹, LIU Bin², LI Sheng-jie³, DONG Shi^{1,Corresponding Author}

1. Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, College of Life Science, Tianjin Normal University, Tianjin 300387;
2. Tianjin Tianxiang Aquatic Company Limited, Tianjin 301500;
3. Pear River Fisheries Institute, Chinese Academy of Fishery Science, Guangzhou 510380

Received:2012-05-15 Online:2012-09-20 Published:2019-11-12

Abstract

Abstract: Using horizontal starch gel electrophoresis method,the 12 isozymes of muscles,liver,kidney,heart,eye,brain and gill in two individuals of largemouth bass were examined. The seven isozumes of AAT,GPI,IDH,LDH,MDH,ME and PGM were useful for determining genotypes of largemouth bass, these seven isozymes of two subspecies of northern largemouth bass M. salmoides salmoides and Florida largemouth bass M. salmoides floridanus were examined. The 13 loci were observed, except AAT-1*, the polymorphic locus at 12 loci among individuals within subspecies in two subspecies were not found. The heterozygosities of Ho and He were zero. The genes of two subspecies at two loci of IDH-1* and MDH-1* were different, and this is useful for identifying the two subspecies. The Nei’s genetic distance between the two subspecies was 0.182 3.

Key words: largemouth bass（Micropterus salmoides）, isozyme, genetic diversity

CLC Number:

Q344.5

ZHANG Da-li, YANG Qiang, HAO Jun, LIU Bin, LI Sheng-jie, DONG Shi. Isozyme Analysis of Northern and Florida Subspecies of Largemouth Bass Micropterus salmoides[J]. Journal of Tianjin Agricultural University, 2012, 19(3): 8-12.

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Isozyme Analysis of Northern and Florida Subspecies of Largemouth Bass Micropterus salmoides

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