生长相关的微卫星标记在镜鲤繁殖群体中的验证

Journal of Tianjin Agricultural University ›› 2012, Vol. 19 ›› Issue (3): 13-18.

• Researches and Scientific Notes • Previous Articles Next Articles

Verification of Microsatellite Markers Correlated with Growth Trait in Mirror Carp Cyprinus carpio L.

LU Cui-yun^1,3, CAO Ding-chen¹, LI Chao¹, LI Chong-wen², SUN Xiao-wen^{1,Corresponding Author}

1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
2. Tianjin Tianxiang Aquatic Company Limited, Tianjin 301500, China;
3. College of Fisheries and life Science, Shanghai Ocean University, Shanghai 201306, China

Received:2012-05-17 Online:2012-09-20 Published:2019-11-12

Abstract

Abstract: Correlation between Genotypes of eight microsatellite loci and growth trait was analyzed and verified using mirror carp breeding population with large samples. Steady and clear DNA bands were amplified in 8 microsatellite markers with the size ranged from 100 to 322 bp. Thirty-three alleles and 75 genotypes were detected. The correlation was analyzed using data of body weight, body length and genotypes. The results show that HLJ343 had a significant impact on body weight and length. In addition, there were three loci, HLJ319, HLJ302 and HLJE339, and they had a significant impact on body length. This analysis reveals that the 4 QTL loci had correlation with body weight and length in large samples of mirror carp. Next, it can guide the breeding program according to the dominant genotype. And then, the researches in molecular marker assistant breeding selection will be carried out based on the QTL results.

Key words: microsatellite marker, body weight, body length, Cyprins carpio L.

CLC Number:

S968

LU Cui-yun, CAO Ding-chen, LI Chao, LI Chong-wen, SUN Xiao-wen. Verification of Microsatellite Markers Correlated with Growth Trait in Mirror Carp Cyprinus carpio L.[J]. Journal of Tianjin Agricultural University, 2012, 19(3): 13-18.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/

http://xuebao.tjau.edu.cn/EN/Y2012/V19/I3/13

References

[1] 孙效文,梁利群. 鲤鱼的遗传连锁图谱(初报)[J]. 中国水产科学,2000,7(1):1-6.
[2] 孙效文,鲁翠云,匡友谊,等. 镜鲤两个繁殖群体的遗传结构和几种性状的基因型分析[J]. 水产学报,2007,31(3):273-279.
[3] 张研,梁利群,常玉梅,等. 鲤鱼体长性状的QTL定位及其遗传效应分析[J]. 遗传,2007,29(10):1243-1248.
[4] 顾颖,曹顶臣,张研,等. 鲤与生长性状相关的EST-SSRs标记筛选[J]. 中国水产科学,2009,16(1):15-22.
[5] 杨晶, 张晓峰, 储志远, 等. 鲤的微卫星标记与体质量、体长、体高和吻长的相关分析[J]. 中国水产科学,2010,17(4):721-730.
[6] 储志远,张晓峰,曹柱,等. 鲤回交群体4种生长性状的相关性分析[J]. 水产学报,2011,35(1):10-19.
[7] 郑先虎,曹顶臣,匡友谊,等. 镜鲤体质量和体长的QTL定位研究[J]. 中国水产科学,2012,19(2):189-195.
[8] 孙效文,鲁翠云,贾智英,等. 水产动物分子育种研究进展[J]. 中国水产科学,2009,16(6):981-990.
[9] 鲁翠云,曹顶臣,孙效文,等. 微卫星分子标记辅助镜鲤家系构建[J]. 中国水产科学,2008,15(6):893-901.
[10] 张研. 鲤鱼(Cyprinus carpio L.)遗传图谱构建及其生长相关性状的QTL分析[D]. 上海:上海海洋大学,2008.
[11] 李鸥,曹顶臣,张研,等. 利用EST-SSR分子标记研究鲤的饲料转化率性状[J]. 水产学报,2009,33(4):624-631.
[12] Botstein D,White R I,Skolnich M.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am J Human Genet,1980,32:314-331.
[13] 常玉梅,孙效文. 水产养殖动物遗传连锁图谱及QTL定位研究进展[J]. 动物学研究,2006,27(5):533-540.
[14] 孙效文. 鱼类分子育种学[M]. 北京:海洋出版社,2010:188-199.
[15] 侯宁,张研,鲁翠云,等. 微卫星DNA标记分析德国镜鲤的遗传潜力[J]. 遗传,2007,29(12):1509-1518.
[16] 孙新,魏振邦,孙效文,等. 镜鲤繁殖群体的遗传结构及微卫星标记与经济性状的相关性分析[J]. 遗传,2008,30(3):359-366.
[17] 孙效文,鲁翠云,曹顶臣,等. 镜鲤体重相关分子标记与优良子代的筛选和培育[J]. 水产学报,2009,33(2):177-181.

Verification of Microsatellite Markers Correlated with Growth Trait in Mirror Carp Cyprinus carpio L.

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments