藏鸡源高抑菌活性乳酸菌筛选鉴定及其对鼠伤寒沙门菌感染小鼠的保护效应

doi:10.19640/j.cnki.jtau.2026.05.009

Journal of Tianjin Agricultural University ›› 2026, Vol. 33 ›› Issue (5): 62-67.doi: 10.19640/j.cnki.jtau.2026.05.009

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Screening and identification of lactic acid bacteria with high antimicrobial activity from Tibetan chicken and its protective effects against Salmonella Typhimurium infection in mice

SunYucai¹, Liu Tingting¹, Jike Nawei¹, Jiang Xiaotiao², Feng Weidong³, Rao Kaiqing^{1,Corresponding Author}

1. Key Laboratory of Animal Medicine at Southwest Minzu University of Sichuan Province, Chengdu 610041, China;
2. Department of Agriculture, Animal Husbandry and Rural Affairs, Shiqu County, Ganzi Tibetan Autonomous Prefecture, Ganzi Tibetan Autonomous Prefecture 626000, Sichuan Province, China;
3. Animal Husbandry Science Research Institute of Ganzi Tibetan Autonomous Prefecture, Ganzi Tibetan Autonomous Prefecture 626000, Sichuan Province, China

Received:2025-07-16 Published:2026-11-02

Abstract

Abstract: This study aimed to isolate and characterize bacteriocin-producing lactic acid bacteria strains from Tibetan chicken samples, screen bacteriocin-producing lactic acid bacteria with high antibacterial activity, and explore their ability to antagonize Salmonella Typhimurium in mice and its effect on the phagocytosis of mouse peritoneal macrophages. In this experiment, 69 strains of lactic acid bacteria isolated from Tibetan chicken manure were screened by Oxford cup method, and 6 strains of lactic acid bacteria were obtained, which could inhibit both indicator Escherichia coli and Staphylococcus aureus, and still had strong antibacterial activity after excluding the effects of organic acids, hydrogen peroxide and protease K. The 6 strains were identified by 16S rRNA as 4 strains of Lactobacillus salivarius, 1 strain of Lactobacillus plantarum and 1 strain of Lactobacillus crispatus. In this experiment, Lactobacillus plantarum S1 and Lactobacillus crispatus S2, which had the strongest inhibition effect on indicator bacteria, were selected as the research objects of the subsequent experiment. Both Lacotabacillus plantarum S1 and Lactobacillus crispatus S2 produced bacteriocins, remained viable in the presence of bile salts and at different pH values（2.0, 3.0）, and were sensitive to antibiotics. The test strains S1 and S2 were administered to mice by oral gavage prior to infection with Salmonella Typhimurium. Both strains reduced the number of S. Typhimurium in the liver and spleen of mice and increased the phagocytic rate and phagocytic index of mouse peritoneal macrophages. In conclusion, L. plantarum S1 and L. crispatus S2 isolated from Tibetan chicken feces exhibited high antibacterial activity and conferred protective effects against Salmonella Typhimurium infection in mice.

Key words: bacteriocin, Tibetan chicken, Lactobacillus plantarum, Lactobacillus crispatus, in vivo antibacterial protective effect

CLC Number:

SunYucai, Liu Tingting, Jike Nawei, Jiang Xiaotiao, Feng Weidong, Rao Kaiqing. Screening and identification of lactic acid bacteria with high antimicrobial activity from Tibetan chicken and its protective effects against Salmonella Typhimurium infection in mice[J]. Journal of Tianjin Agricultural University, 2026, 33(5): 62-67.

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URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2026.05.009

http://xuebao.tjau.edu.cn/EN/Y2026/V33/I5/62

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Screening and identification of lactic acid bacteria with high antimicrobial activity from Tibetan chicken and its protective effects against Salmonella Typhimurium infection in mice

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