Abstract: Strain ZMN-3 is a new exopolysaccharide-producing strain of Massilia isolated from desertified soil. Recently, the classification of some members of this genus in GTDB has been revised, but the classification of these members has not been analyzed from other taxonomic perspectives. In order to clarify the membership relationship of ZMN-3 related genera and explore its potential biological functions, this study adopted polygene evolutionary tree analysis, gene annotation function analysis and comparative genomic analysis, and predicted the secondary metabolite gene cluster. The results showed that strains ZMN-3 and Pseudoduganella plicata DSM 17505 have good collinearity. The combination of these results with MLSA and physicochemical analysis supports the reclassification of strain ZMN-3 from the Massilia genus to the Pseudoduganella genus. The genome of strain ZMN-3 had a size of 6 337 491 bp, a GC content of 66.5%, and contained 5 541 coding genes. The annotation results of the COG, GO, and KEGG databases show that strain ZMN-3 has the potential to synthesize various types of compounds for soil remediation. The bioinformatics analysis of strain ZMN-3 to explore its potential biological functions can provide data support and theoretical basis for the subsequent development and utilization of strain ZMN-3.

Key words: Pseudoduganella, whole genome sequencing, gene function annotation, comparative genomic analysis, bioinformatics analysis