红豆杉多糖对IBDV B87株感染DF-1细胞的抗病毒作用

doi:10.19640/j.cnki.jtau.2026.03.005

Journal of Tianjin Agricultural University ›› 2026, Vol. 33 ›› Issue (3): 25-29.doi: 10.19640/j.cnki.jtau.2026.03.005

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Antiviral effects of taxus polysaccharides on IBDV B87 strain infected DF-1 cells

Zhang Xinli, Ding Yuan, Zhang Xiying, Wang Tianyu, Li Songyue, Zhao Wei^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received:2025-03-07 Online:2026-06-30 Published:2026-06-30

Abstract

Abstract: In order to investigate the activity of taxus polysaccharides（TP）against chicken infectious bursal disease virus（IBDV）and its mechanism of action, experiments were carried out to evaluate the effect of TP on the infection of DF-1 cells with IBDV B87 strain on an in vitro fibroblast cell culture system by three modes of action: direct inactivation, inhibition, and blockade. The antiviral activity of the taxus polysaccharides was detected by using cytopathic inhibition assay and CCK-8. The results showed that the maximum safe concentration of taxus polysaccharide was 125 μg/mL, at this concentration, taxus polysaccharides had a certain direct inactivation effect on B87 strain, and the best antiviral effect was achieved by pretreatment for 1 h. However, it could not effectively prevent the virus adsorption. This suggests that the taxus polysaccharide can play an antiviral role by directly inactivating or blocking the virus from invading the cells.

Key words: TP, IBDV, immunosuppression, immune regulation

CLC Number:

S852.6

Zhang Xinli, Ding Yuan, Zhang Xiying, Wang Tianyu, Li Songyue, Zhao Wei. Antiviral effects of taxus polysaccharides on IBDV B87 strain infected DF-1 cells[J]. Journal of Tianjin Agricultural University, 2026, 33(3): 25-29.

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Antiviral effects of taxus polysaccharides on IBDV B87 strain infected DF-1 cells

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