基于γ-Fe2O3磁性纳米微球的过氧化物模拟酶研究

doi:10.19640/j.cnki.jtau.2022.01.010

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (1): 47-51.doi: 10.19640/j.cnki.jtau.2022.01.010

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Studies on peroxidase mimics based on γ-F₂O₃ magnetic nanoparticles

Yang Jiao¹, Qin Mingshan¹, Lü Haijun¹, Yan Xuetao¹, Yang Guijun², Yang Linyan^{1,Corresponding Author}

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Key Laboratory of Biological Feed Additive Enterprises, Tianjin 300383, China

Received:2021-12-08 Online:2022-03-31 Published:2022-04-14

Abstract

Abstract: This article intends to study whether γ-Fe₂O₃-based magnetic nanoparticles can have the catalytic properties of peroxidase mimics by chemical modification. By preparing SiO₂-coated γ-Fe₂O₃ magnetic nanoparticles, and then adopting covalent modification methods, γ-Fe₂O₃@SiO₂ magnetic nanoparticles were modified with glucose oxidase (GOD), γ- Fe₂O₃@SiO₂-NH₂-COOH-GOD magnetic nanoparticles were obtained, and then γ-Fe₂O₃, γ-Fe₂O₃@SiO₂, γ-Fe₂O₃@SiO₂-NH₂, γ-Fe₂O₃@SiO₂-NH₂-COOH and γ-Fe₂O₃@SiO₂-NH₂-COOH-GOD magnetic nanoparticles were used for structural confirmation. The results showed that the amination, carboxylation and GOD-modification of the magnetic nanoparticles were successful. After measuring with a fluorescence spectrophotometer, γ-Fe₂O₃@SiO₂-NH₂-COOH-GOD was proved to have the catalytic properties of a peroxidase mimicking enzyme, which can catalyze the reaction between H₂O₂ and TMB. However, if the glucose decomposition concentration is too high, the enzyme quenching will also occur.

Key words: γ-Fe₂O₃, GOD, magnetic nanoparticles, peroxidase mimics, functional modification

CLC Number:

Q658.2

Yang Jiao, Qin Mingshan, Lü Haijun, Yan Xuetao, Yang Guijun, Yang Linyan. Studies on peroxidase mimics based on γ-F₂O₃ magnetic nanoparticles[J]. Journal of Tianjin Agricultural University, 2022, 29(1): 47-51.

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Studies on peroxidase mimics based on γ-F₂O₃ magnetic nanoparticles

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Studies on peroxidase mimics based on γ-F₂O₃ magnetic nanoparticles