Abstract: With Chlorella. vulgaris as the test organism, four homogenizate methods ,liquid nitrogen method, grinding method, mechanical method, and repeated freeze-thaw method were studied. The homogenation with the above methods was used to determined the SOD activity and GSH content, which aimed to select a method with high crushing efficiency, convenience, and accurate determination of physiological and biochemical indicators. The results showed that the crushing rates of the four methods were 81.43%, 100%, 92.41%, 56.54%, and the SOD activity were 47.06, 48.58, 0, 25.05 U/mgprot. There was no significant difference between liquid nitrogen method and grinding method（p>0.05）; GSH content were 34.42, 24.52, 87.37, 38.75 mgGSH/gprot; There was no significant difference apart of mechanical method（p>0.05）. The above results indicated that the grinding method and liquid nitrogen method have a high crushing rate, and accurate measurement of the biochemical indicators. However, grinding method consumed too much labor and cannot process a large number of samples, so the liquid nitrogen method was considered to be the best solution for preparing microalgal cell homogenation.

Key words: Chlorella. vulgaris, homogenate methods, crushing rate, biochemical indicators