PCL生物反应器中催化合成乙酸正戊酯

doi:10.19640/j.cnki.jtau.2020.01.015

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (1): 65-69.doi: 10.19640/j.cnki.jtau.2020.01.015

• Researches and Scientific Notes • Previous Articles Next Articles

Synthesis of n-amyl acetate in a PCL bioreactor

REN De-zhong¹, CONG Fang-di^{1,2,Corresponding Author}, WANG Xiao-hong¹, ZHANG Meng¹, WANG Xin-xin², ZHANG Shu-lin², LUO Wei¹

1. College of Basic Sciences, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Key Lab of Aqua-Ecology and Aquaculture, Tianjin 300384, China

Received:2019-05-19 Published:2020-04-01

Abstract

Abstract: In order to improve the efficiency of lipase-catalyzed synthesis of esters and optimize the catalytic conditions, Pseudomonas cepacia lipase was immobilized on absorbent cotton by physical adsorption to form a simple bioreactor with column bottle or titrator tube, and used to catalyze the reaction of vinyl acetate and n-amyl alcohol to synthesize n-amyl acetate. The effects of temperature, substrate ratio and solvent dosage on transesterification were studied, and also the stability, configuration and usage method of bioreactor were explored. It was found that the conversion of n-amyl alcohol to n-amyl acetate could achieve at 99% and 97% in an unshaken column bottle bioreactor at room temperature 37 ℃ and 25 ℃ after 24 hours, respectively. And the conversion also reached at 77% in a static tube bioreactor at room temperature after 5 h. It can be seen that the prepared enzymatic bioreactor can effectively catalyze the synthesis of n-amyl acetate under green and low carbon conditions.

Key words: n-amyl acetate, Pseudomonas cepacia lipase, absorbent cotton, static state, conversion

CLC Number:

TS201.3

REN De-zhong, CONG Fang-di, WANG Xiao-hong, ZHANG Meng, WANG Xin-xin, ZHANG Shu-lin, LUO Wei. Synthesis of n-amyl acetate in a PCL bioreactor[J]. Journal of Tianjin Agricultural University, 2020, 27(1): 65-69.

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Synthesis of n-amyl acetate in a PCL bioreactor

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