天津农学院学报 ›› 2014, Vol. 21 ›› Issue (2): 13-17.

• 研究与简报 • 上一篇    下一篇

镉对黄金鲫部分生化指标的影响

孙朦朦1, 黄亚冬2, 孙学亮1, 郭永军1, 陈成勋1, 邢克智1,通信作者   

  1. 1. 天津农学院 水产学院 天津市水产生态及养殖重点实验室,天津 300384;
    2. 天津海升水产养殖有限公司,天津 300272
  • 收稿日期:2014-01-10 发布日期:2019-10-21
  • 通讯作者: 邢克智(1956-),男,天津市人,教授,学士,主要从事养殖水域生态学研究。E-mail:kzxing6668@126.com。
  • 作者简介:孙朦朦(1988-),女,河南周口人,硕士在读,主要从事水产养殖方向研究。E-mail:smmxny@163.com。
  • 基金资助:
    基金资助:天津市星火计划项目“黄金鲫良种繁育及健康养殖技术示范”(09ZHXHNC05100); 国家星火计划项目“黄金鲫工厂化育苗及健康养殖技术示范”(2010GA610007); 天津市农业科技示范推广项目“黄金鲫良种繁育及健康养殖技术推广”(201001010); 国家级科技计划项目“水产品健康养殖、加工与质量安全控制产业化示范”(2013GA610003)

Effects of Cadmium on Some Biochemical Indicators of Golden Carp

SUN Meng-meng1, HUANG Ya-dong2, SUN Xue-liang1, GUO Yong-jun1, CHEN Cheng-xun1, XING Ke-zhi1,Corresponding Author   

  1. 1. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
    2. Tianjin Haisheng Aquaculture Co. Ltd, Tianjin 300272, China
  • Received:2014-01-10 Published:2019-10-21

摘要: 为研究腹腔注射Cd2+对黄金鲫血液生化指标的影响,试验将300尾健康黄金鲫随机分为4组,分别为对照K组、A组、B组和C组,其中Cd2+的注射浓度依次为0、1.25、2.50、3.75 mg/kg,测定注射后0、1、3、5、7 d时的血清超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)和丙二醛(MDA)的变化。结果表明,鱼体血液中各指标的变化与注射镉元素密切相关。与对照组相比,A组血清中SOD活力在前3天显著升高(P<0.05),在第5、7天时差异不显著(P>0.05),B组和C组中SOD活力在整个试验期间显著降低(P<0.05);A组、B组和C组血清中CATACP活性和MDA含量与对照相比均显著增加(P<0.05);A组AKP活性在第1天时与对照组无显著差异,之后显著降低,B组和C组均显著低于对照组(P<0.05)。

关键词: 黄金鲫, 血清, 镉, 生化指标

Abstract: :It is aimed to investigate the effects of intraperitoneal injection of Cd2 + on serum biochemical indictors of golden carp. 300 healthy golden carps were randomly divided into 4 groups with 3 replicates per group, namely the control group, A group, B group, C group and Cd2 + concentration of these groups were 0, 1.25, 2.50 and 3.75 mg/kg in turn, serum superoxide dismutase(SOD), acid phosphatase(ACP), alkaline phosphatase(AKP), catalase(CAT), malondialdehyde(MDA)contents among these different groups were determined at 0, 1, 3, 5, 7 d after injection respectively. The results show as follows. The changes of serum indicators were closely related with injecting cadmium elements. Compared with the control group, serum SOD activity in A group was significantly higher in the first three days(P<0.05), and there was no significant difference at 5, 7 days, while the serum SOD activity in group B and group C during the whole trial period was significantly lower than that of the control group (P<0.05). The serum CAT activity, ACP activity and MDA content in group A, group B and group C were significantly higher than those of the control group(P<0.05). AKP activity in A group compared with the control group had no significant difference in the first day, and then decreased significantly, AKP activity in group B and group C were significantly lower than that of the control group(P<0.05).

Key words: golden carp, serum, cadmium, biochemical indicator

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