鞘氨醇单胞菌TP-3中基因spsK的克隆及生物信息学分析

天津农学院学报 ›› 2013, Vol. 20 ›› Issue (3): 1-4.

• 研究与简报 • 下一篇

鞘氨醇单胞菌TP-3中基因spsK的克隆及生物信息学分析

黄海东, 詹欣娜, 李娜

天津农学院农学系,天津 300384

收稿日期:2013-04-15 发布日期:2019-10-21
作者简介:黄海东（1972-）,男,浙江台州人,副教授,博士,主要从事资源细菌及工程方面的研究。E-mail：hdhuang@tjau.edu.cn。
基金资助:
天津市自然科学基金重点项目"新型鞘氨醇胶合成关键基因的研究"（11JCZDJC16600）

Cloning and Bioinformatics Analysis of spsK Gene from Sphingomonas sanxanigenens sp. nov. TP-3

HUANG Hai-dong, ZHAN Xin-na, LI Na

Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China

Received:2013-04-15 Published:2019-10-21

摘要/Abstract

摘要： 以自杀性质粒pUT携带的mini-Tn5转座子对菌株TP-3进行转座诱变,从转座突变库中筛选到不能合成产物鞘氨醇胶Ss的突变株,进而采用Tn5外向扩增与LP-RAPD相结合的方法,得到糖基转移酶编码基因spsK的801 bp序列全长。生物信息学分析表明,其氨基酸序列与迪特胶以及S88合成途径中的糖基转移酶同源性最高,达47%;SpsK蛋白具有典型的G-loop和DXD保守模式,属于糖基转移酶的GT-A家族。

关键词: 鞘氨醇单胞菌, 鞘氨醇胶Ss, 糖基转移酶, 生物信息学

Abstract: Strian TP-3 was induced by transposon mini-Tn5 on suicide plasmid pUT, and a mutant strain which could not produce sphingan Ss was screened. The spsK gene of TP-3 was obtained by the method of Tn5 flanking PCR and LP-RAPD. The spsK gene coding region includes 801 bp and encodes glycosyl transferase. The bioinformatics analysis reveals that spsK from strain TP-3 showed the highest of 47% amino acid sequence identity with glycosyl transferase from biosynthesis of diutan and S88 sphingan. SpsK protein from strain TP-3 belongs to the glycosyl transferase GT-A family with the typical motif of G-loop and DXD.

Key words: Sphingomonas sp., sphingan Ss, glycosyl transferase, bioinformatics

中图分类号:

Q781

黄海东, 詹欣娜, 李娜. 鞘氨醇单胞菌TP-3中基因spsK的克隆及生物信息学分析[J]. 天津农学院学报, 2013, 20(3): 1-4.

HUANG Hai-dong, ZHAN Xin-na, LI Na. Cloning and Bioinformatics Analysis of spsK Gene from Sphingomonas sanxanigenens sp. nov. TP-3[J]. Journal of Tianjin Agricultural University, 2013, 20(3): 1-4.

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鞘氨醇单胞菌TP-3中基因spsK的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of spsK Gene from Sphingomonas sanxanigenens sp. nov. TP-3

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