天津农学院学报 ›› 2018, Vol. 25 ›› Issue (1): 38-43.doi: 10.19640/j.cnki.jtau.2018.01.009

• 研究与简报 • 上一篇    下一篇

pH对淡水小球藻叶绿素荧光参数及生长的影响

薄香兰, 刘兴, 柴英辉, 窦勇, 高金伟, 贾旭颖, 周文礼通信作者   

  1. 天津农学院 水产学院 天津市水产生态及养殖重点实验室,天津 300384
  • 收稿日期:2017-09-20 出版日期:2018-03-20 发布日期:2019-11-05
  • 通讯作者: 周文礼(1969-),男,研究员,博士,主要从事微藻资源化利用与生态学研究。E-mail:saz0908@126.com。
  • 作者简介:薄香兰(1993-),女,硕士在读,主要从事微藻资源化利用和水生态学研究。E-mail:1065817937@qq.com。
  • 基金资助:
    天津市水产产业技术体系创新团队项目(ITTFRS2017005); 天津市科技重大专项与工程项目(15ZXBFNC00120); 卫星海洋环境动力学国家重点实验室开放基金项目(SOED1419); 农业部南海渔业资源开发利用重点实验室开放基金项目(FREU2015-04); 天津农学院科学研究计划项目(2013NO8)

Effects of pH on chlorophyll fluorescence parameters and growth of Chlorella vulgaris

BO Xiang-lan, LIU Xing, CHAI Ying-hui, DOU Yong, GAO Jin-wei, JIA Xu-ying, ZHOU Wen-liCorresponding Author   

  1. Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2017-09-20 Online:2018-03-20 Published:2019-11-05

摘要: 以小球藻为研究对象,研究不同pH对其叶绿素荧光、叶绿素含量和细胞密度的影响,以期找到小球藻最适生长的pH值,为小球藻的集约化培养提供基础资料。结果表明:不同pH对小球藻的叶绿素荧光、叶绿素含量和细胞密度有显著影响,pH值为9时最大光能转化速率(Fv/Fm)、实际光能转化效率(ΦPSⅡ)和量子效率Yield均呈下降趋势,pH值为9时潜在活力(Fv/F0)、相对电子转化速率ETR、叶绿素CHL及小球藻密度上升趋势最小,pH为7时叶绿素含量和细胞密度均高于其他实验组,其值分别为1 613.05 μg/L、1.13×107cells/mL,pH9处理组的叶绿素含量和细胞密度最低,其值为883.82 μg/L、6.77×106cells/mL。小球藻最适生长pH值为7,pH为9时会显著抑制小球藻生长。

关键词: 淡水小球藻, 叶绿素荧光, 叶绿素含量, 细胞密度, pH

Abstract: To determine the optimum pH conditions and provide basic information for intensive production of Chlorella vulgaris, the effects of different pH values on chlorophyll fluorescence, chlorophyll content and cell density during its culture process have been studied. The results show that when pH is 9 the maximum energy conversion rate(Fv/Fm), the actual photochemical efficiency(ΦPSⅡ)and the quantum efficiency(Yield)decreased; meanwhile, the potential energy(Fv/F0), relative electronic conversion rate(ETR), chlorophyll content(CHL)and the density of Chlorella increasing trend was minimum. When pH is 7, chlorophyll fluorescence are higher than the other groups with the values 1 613.05 µg/L, 1.13×107 cells/mL respectively. The chlorophyll content and cell density of pH 9 treatment group were 883.82 µg/L and 6.77×106cells/mL. The optimum growth pH of Chlorella is 7, and pH 9 can obviously inhibit the growth of Chlorella.

Key words: Chlorella vulgaris, chlorophyll fluorescence, chlorophyll content, cell density, pH

中图分类号: