盐单胞属新种Halomonas binhaiensis Y2R2的基因组分析与功能挖掘

doi:10.19640/j.cnki.jtau.2025.04.002

天津农学院学报 ›› 2025, Vol. 32 ›› Issue (4): 9-17.doi: 10.19640/j.cnki.jtau.2025.04.002

盐单胞属新种Halomonas binhaiensis Y2R2的基因组分析与功能挖掘

张亚茹^a, 张钶^a, 韩旭^a, 陈帅君^a, 李晓雁^b, 黄海东^{a,通信作者}

天津农学院 a.农学与资源环境学院,b.食品科学与生物工程学院,天津 300392

收稿日期:2024-03-06 发布日期:2025-09-02
通讯作者: 黄海东（1972—）,男,教授,博士,主要从事微生物多糖的资源开发与应用方面的研究。E-mail：hdhuang@tjau.edu.cn。
作者简介:张亚茹（2002—）,女,本科在读,主要从事微生物资源开发与利用方面的研究。E-mail：zhangyaru0228@163.com。
基金资助:
国家级大学生创新创业训练计划项目（202210061003）

Genome analysis and functional mining of Halomonas binhaiensis Y2R2, a novel species of Halomonas

Zhang Yaru^a, Zhang Ke^a, Han Xu^a, Chen Shuaijun^a, Li Xiaoyan^b, Huang Haidong^{a,Corresponding Author}

Tianjin Agricultural University, a. College of Agronomy and Resource Environment, b. College of Food Science and Bioengineering, Tianjin 300392, China

Received:2024-03-06 Published:2025-09-02

摘要/Abstract

摘要： Halomonas binhaiensis Y2R2是从天津滨海盐碱地中分离得到的具有溶磷功能的盐单胞菌属（Halomonas）新种,为发掘菌株Y2R2的菌种信息,采取基因组功能注释与比较基因组学分析的方法,对其可能的次级代谢产物合成基因簇进行预测,探究该菌株的潜在功能。菌株Y2R2的GO、COG和KEGG等数据库功能注释结果表明,菌株Y2R2参与碳水化合物代谢和氨基酸代谢的相关基因较多,分别有341个和336个。通过与3个近缘模式菌株的比较基因组分析,发现有3 623个共有的基因家族基因,663个特有基因。其中,Y2R2与黄河盐单胞菌（Halomonas huangheensis）NTU-107的同源性最高;预测得到多个菌株Y2R2次级代谢产物合成基因簇,包括四氢嘧啶（Ectoine）和细菌萜类化合物Sodorifen的合成基因簇。其中,四氢嘧啶合成基因簇与地中海甲壳虫（Methylophaga thalassica）的四氢嘧啶合成基因簇相似度高达75%。通过生物信息学分析,探究菌株Y2R2适应高渗透压环境和溶磷的机理,挖掘菌株在盐碱地植物促生方面的潜在功能,为盐单胞菌属新种菌株Y2R2在滨海盐碱地改良领域的应用提供技术支持。

关键词: 盐单胞菌, 溶磷菌, 功能注释, 基因功能挖掘, 次级代谢产物

Abstract: Halomonas binhaiensis Y2R2 is a novel species of the genus Halomonas with a phosphate-solubilizing function, which was isolated from Tianjin coastal saline-alkali land. In this paper, the genome function of strain Y2R2 was annotated and compared, and the gene cluster of secondary metabolites synthesis was predicted to explore its potential functions. Functional annotation of GO, COG and KEGG databases of strain Y2R2 showed that there were 341 and 336 genes involved in carbohydrate metabolism and amino acid metabolism, respectively. Comparative genomic analysis between Y2R2 and three closely related model strains showed that there were 3 623 gene families and 663 unique genes, among which the homology with Halomonas huangheensis NTU-107 was the highest. A number of strain Y2R2 secondary metabolite synthesis gene clusters were predicted, including tetrahydropyrimidine and bacterial terpenoid sodorifen. The similarity between tetrahydropyrimidine synthesis gene cluster and that of Methylophaga thalassica was as high as 75%. Through bioinformatics analysis, the mechanism of strain Y2R2 adapting to high osmotic pressure environment and dissolving phosphorus was explored, and the potential function of strain Y2R2 in promoting the growth of saline-alkali plants was explored, which provided support for the application of novel species Y2R2 of Halomonas in coastal saline-alkali land improvement.

Key words: Halomonas, phosphate-solubilizing bacteria, functional annotation, gene function mining, secondary metabolites

中图分类号:

Q939.11⁺2

张亚茹, 张钶, 韩旭, 陈帅君, 李晓雁, 黄海东. 盐单胞属新种Halomonas binhaiensis Y2R2的基因组分析与功能挖掘[J]. 天津农学院学报, 2025, 32(4): 9-17.

Zhang Yaru, Zhang Ke, Han Xu, Chen Shuaijun, Li Xiaoyan, Huang Haidong. Genome analysis and functional mining of Halomonas binhaiensis Y2R2, a novel species of Halomonas[J]. Journal of Tianjin Agricultural University, 2025, 32(4): 9-17.

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http://xuebao.tjau.edu.cn/CN/Y2025/V32/I4/9

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盐单胞属新种Halomonas binhaiensis Y2R2的基因组分析与功能挖掘

Genome analysis and functional mining of Halomonas binhaiensis Y2R2, a novel species of Halomonas

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