蛹虫草菌侵染柞蚕蛹条件的研究

天津农学院学报 ›› 2017, Vol. 24 ›› Issue (4): 48-51.

蛹虫草菌侵染柞蚕蛹条件的研究

张博¹, 孙宁², 孙永健^{1,通信作者}, 张颖¹, 路雨¹, 何媛媛¹

1.天津天狮学院生物与食品工程学院,天津 301700;
2. 天津农学院农学与资源环境学院,天津 300384

收稿日期:2017-03-22 出版日期:2017-12-31 发布日期:2019-10-15
通讯作者: 孙永健（1983-）,男,天津市人,讲师,硕士,研究方向为药食两用真菌栽培及生物活性物质分离筛选。E-mail：sunyongjian 2008@126.com。
作者简介:张博（1994-）,女,河北沧州人,本科在读,研究方向为生物工程。E-mail：2878290843@qq.com。
基金资助:
天津天狮学院科研项目“柞蚕蛹虫草培育关键技术的研究”（K14004）; 天津市大学生创新创业训练计划项目“优质桑蚕蛹虫草培育关键技术的研究”（201610859003）

Study on Conditions of Antheraea pernyi Pupa Infected by Cordyceps militaris（L.）Link

ZHANG Bo¹, SUN Ning², SUN Yong-jian^{1,Corresponding Author}, ZHANG Ying¹, LU Yu¹, HE Yuan-yuan¹

1. Biotechnology and Food Engineering Department, Tianjin Tianshi College, Tianjin 301700, China;
2. College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300384, China

Received:2017-03-22 Online:2017-12-31 Published:2019-10-15

摘要/Abstract

摘要： 分别从菌种处理、接种方式、寄主三个角度研究了蛹虫草菌侵染柞蚕蛹的条件,结果表明,用添加70%柞蚕蛹浸出汁制作蛹虫草菌的液体培养基对菌种进行活化培养,以注射的方式接种于灭菌后（121℃,20 min）的柞蚕蛹体内,培养出的子实体最好,蛹体完全僵化所需时间缩短至6 d,僵化率及发草率分别提高至98.0%和99.3%。

关键词: 蛹虫草, 柞蚕蛹, 侵染

Abstract: This research was carried out from three aspects, including strain, inoculation and host, to study the conditions of Antheraea pernyi pupa infected by Cordyceps militaris（L.）Link. The result shows that add 70% leaching juice of the pupa into medium for activation of strain and inoculate to sterilized（121 ℃, 20 min）pupa by injection, can make the fruiting body of Cordyceps militaris（L.）Link achieve the best effect. The time of pupa become rigid can be shortened to 6 days and the rate of rigidity and germination can be increased to 98.0% and 99.3%, respectively.

Key words: Cordyceps militaris（L.）Link, Antheraea pernyi Pupa, infection

中图分类号:

S567.35

张博, 孙宁, 孙永健, 张颖, 路雨, 何媛媛. 蛹虫草菌侵染柞蚕蛹条件的研究[J]. 天津农学院学报, 2017, 24(4): 48-51.

ZHANG Bo, SUN Ning, SUN Yong-jian, ZHANG Ying, LU Yu, HE Yuan-yuan. Study on Conditions of Antheraea pernyi Pupa Infected by Cordyceps militaris（L.）Link[J]. Journal of Tianjin Agricultural University, 2017, 24(4): 48-51.

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