特异性靶基因在弧菌PCR快速检测中的应用

天津农学院学报 ›› 2015, Vol. 22 ›› Issue (3): 49-53.

特异性靶基因在弧菌PCR快速检测中的应用

邵蓬¹, 汪笑宇², 徐晓丽¹

1. 天津市水产技术推广站,天津 300221;
2. 天津渤海水产研究所,天津 300457

收稿日期:2015-03-14 发布日期:2015-09-30
通讯作者: 国家农业科技成果转化基金项目“出口级特色水产品健康养殖技术转化与应用”（2014GB2A100511）
作者简介:邵蓬（1982-）,男,山东日照人,工程师,硕士,主要从事水产养殖技术推广、病害及免疫学研究。E-mail: vipshaopeng@ sohu.com。

Application of Specific Target Genes to PCR Rapid Detection for Vibrios

SHAO Peng¹, WANG Xiao-yu², XU Xiao-li¹

1. Tianjin Fishery Technical Extension Station, Tianjin 300221, China;
2. Bohai Fisheries Research Institute of Tianjin, Tianjin 300457, China

Received:2015-03-14 Published:2015-09-30

摘要/Abstract

摘要： 快速、准确地确定弧菌种类能够有效防治弧菌病的发生,利用PCR技术对水产养殖动物的弧菌病进行诊断,通常需要采用靶基因作为基础。近年来,常用的细菌诊断靶基因主要有：16S rDNA、16S-23S rDNA基因间区序列、gyrB基因、dnaJ基因等看家基因和某些弧菌特定的毒力基因,如toxR基因、tdh基因、trh基因、vvhA基因、ctxA基因等。

关键词: 靶基因, 弧菌, PCR, 快速检测

Abstract: Quick and accurate diagnosis of the types of Vibrio can effectively prevent and control the occurrence of Vibrio disease. It is usually needed to use the target genes as the foundation when diagnosing the Vibrio disease of aquatic animals by PCR technology. In recent years, the common target genes for bacterial diagnosis mainly included some house-keeping genes, such as 16S rDNA, 16S-23S intergenic spacer regions, gyrB gene, dnaJ gene and so on, and some specific virulence genes of Vibrio, such as toxR gene, tdh gene, trh gene, vvhA gene, ctxA gene, et al.

Key words: target gene, Vibrio, PCR, rapid detection

中图分类号:

邵蓬, 汪笑宇, 徐晓丽. 特异性靶基因在弧菌PCR快速检测中的应用[J]. 天津农学院学报, 2015, 22(3): 49-53.

SHAO Peng, WANG Xiao-yu, XU Xiao-li. Application of Specific Target Genes to PCR Rapid Detection for Vibrios[J]. Journal of Tianjin Agricultural University, 2015, 22(3): 49-53.

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链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2015/V22/I3/49

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