禽TCR谱系分析的研究进展

天津农学院学报 ›› 2014, Vol. 21 ›› Issue (3): 44-48.

禽TCR谱系分析的研究进展

任超¹, 刘贤勇², 崔丽³

1. 天津农学院动物科学与动物医学学院,天津 300384;
2. 中国农业大学动物医学院,北京 100094;
3. 天津市西青区动物卫生监督所,天津 300380

收稿日期:2014-02-20 出版日期:2014-09-20 发布日期:2019-10-21
通讯作者: 刘贤勇（1981-）,男,湖北巴东人,副教授,博士,主要研究方向为预防兽医学。E-mail：liuxianyong@cau.edu.cn。
作者简介:任超（1986-）,女,天津市人,助理实验师,硕士,主要研究方向为基础兽医学。E-mail：chaoren04050110@163.com。
基金资助:
国家自然科学基金项目“巨型艾美耳球虫感染激发宿主产生的CD8⁺T细胞TCR的谱型分析”（31001060）; 天津农学院科技发展基金项目“ATRA对家禽免疫调节作用的研究”（2013N09）; 天津市高校创新团队建设项目“兽医生物技术”（TD12-5019）

Research Progress of Avian TCR Repertoire Analysis

REN Chao¹, LIU Xian-yong², CUI Li³

1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;
2. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China;
3. Xiqing District Animal Health Authority of Tianjin City, Tianjin 300380, China

Received:2014-02-20 Online:2014-09-20 Published:2019-10-21

摘要/Abstract

摘要： 禽T细胞通过TCR将外部信号转化为内部信号,刺激T细胞增殖分化并发挥效应功能。对TCR谱系进行分析,可以充分展示机体的TCR克隆状况,从而对机体的免疫功能进行全面评估。TCR谱系分析技术已广泛应用于病原体感染、肿瘤、自身免疫性疾病等领域。利于TCR谱系分析技术研究禽类动物在病原体感染和肿瘤疾病发生中TCR克隆的变化情况,能够为病原体的生物学特性以及相关疾病的诊断与防控提供更多的研究资源。

关键词: 禽TCR, 谱系分析, T细胞克隆, 免疫优势性, 多样性

Abstract: Avian T cell can convert the external signal into internal signal via TCR, which can stimulate T cell proliferation and differentiation and play an important role in immune function. TCR clones of the body can be fully shown and evaluate the immune functions comprehensively by TCR repertoire analysis. The technologies of TCR repertoire analysis have been extensively applied to the fields of pathogen infection, tumor, autoimmune disease, and so on. More useful research resources about the biological characteristics of pathogens, diagnosing, preventing and controlling the relevant diseases can be provided by exploring the changes of TCR clones in avian pathogen infection and tumor diseases with using the technologies of TCR repertoire analysis.

Key words: avian TCR, repertoire analysis, T cell clone, immunodominant, diversity

中图分类号:

S857.4

任超, 刘贤勇, 崔丽. 禽TCR谱系分析的研究进展[J]. 天津农学院学报, 2014, 21(3): 44-48.

REN Chao, LIU Xian-yong, CUI Li. Research Progress of Avian TCR Repertoire Analysis[J]. Journal of Tianjin Agricultural University, 2014, 21(3): 44-48.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2014/V21/I3/44

参考文献

[1] Kenneth M,Paul T,Mark W.Janeway’s Immunobiology[M]. The seventh edition. New York: Garland Science,2007:55-298.
[2] Fred D,Bernd K,Karel A S.Avian Immunology[M]. Amsterdam: Elsevier Ltd,2008:91-101.
[3] Chen CL,Cihak J,Lösch U,et al.Differential expression of two T cell receptors,TCR1 and TCR2,on chicken lymphocytes[J]. European Journal of Immunology,1988,18(4):539-543.
[4] Char D,Sanchez P,Chen C L,et al.A third sublineage of avian T cells can be identified with a T cell receptor-3-specific antibody[J]. Journal of Immunology,1990,145(11):3547-3555.
[5] George J F,Cooper M D.Gamma/delta T cells and alpha/beta T cells differ in their developmental patterns of receptor expression and modulation requirements[J]. European Journal of Immunology,1990,20(10):2177-2181.
[6] Bucy R P,Chen C L,Cihak J,et al.Avian T cells expressing gamma delta receptors localize in the splenic sinusoids and the intestinal epithelium[J]. Journal of Immunology,1988,141(7):2200-2205.
[7] Cooper M D,Chen C L,Bucy R P,et al.Avian T cell ontogeny[J]. Advances in Immunology,1991,50:87-117.
[8] Chen C H,Sowder J T,Lahti J M,et al.TCR:A third T-cell receptor in the chicken[J]. PNAS,1989,86:2351-2355.
[9] 金伯全. 细胞和分子免疫学[M]. 2版. 北京:北京科学出版社,2001:109-113.
[10] Kedzierska K,La Gruta N L,Stambas J,et al. Tracking phenotypically and functionally distinct T cell subsets via T cell repertoire diversity[J]. Molecular Immunology,2008,45(3):607-618.
[11] Chothia C,Boswell D R,Lesk A M.The outline structure of the T-cell alpha beta receptor[J]. European Molecular Biology Journal,1988,7(12):3745-3755.
[12] Bentley G A,Mariuzza R A.The structure of the T cell antigen receptor[J]. Annual Review of Immunology,1996(14):563-590.
[13] Davis M M,Boniface J J,Reich Z,et al.Ligand recognition by alpha beta T cell receptors[J]. Annual Review of Immunology,1998(16):523-544.
[14] 李扬秋,林晨,李萡,等. T细胞受体的研究和应用[M].北京:人民卫生出版社,2009:55-92.
[15] Kedzierska K,Turner S J,Doherty P C.Conserved T cell receptor usage in primary and recall responses to an immunodominant influenza virus nucleoprotein epitope[J]. PNAS,2004,101(14):4942-4947.
[16] Li Y,Chen S,Yang L,et al.Clonal expanded TCR Vbeta T cells in patients with APL[J]. Hematology,2005,10(2):135-139.
[17] 李扬秋,陈少华,杨力建,等. CML病人TCR Vβ21寡克隆T细胞及其CDR3序列特点[J]. 免疫学杂志,2000(3):189-192.
[18] Turner S J,Kedzierska K,LaGruta N L,et al. Characterization of CD8+ T cell repertoire diversity and persistence in the influenza A virus model of localized,transient infection[J]. Seminar in Immunology,2004,16(3):179-184.
[19] Fanning S L,Zilberberg J,Stein J,et al.Unraveling graft-versus-host disease and graft-versus-leukemia responses using TCR Vβ spectratype analysis in a murine bone marrow transplantation model[J]. Journal of Immunology,2013,190(1):447-457.
[20] He X Y,Yang W M,Tang W T,et al.TRAV gene expression in PBMCs and TILs in patients with breast cancer analyzed by a DNA melting curve(FQ-PCR)technique for TCR α chain CDR3 spectratyping[J]. Neoplasma,2012,59(6):693-699.
[21] Tuulasvaara A,Baussand J,Laine P,et al.High-sequence diversity and structural conservation in the human T-cell receptor β junctional region during thymic development[J]. European Journal of Immunology,2013,43(8):2185-2193.
[22] Mwangi W N,Beal R K,Powers C,et al.Regional and global changes in TCRαβ T cell repertoires in the gut are dependent upon the complexity of the enteric microflora[J]. Developmental and Comparative Immunology,2010,34(4):406-417.
[23] Mwangi W N,Smith L P,Baigent S J,et al.Clonal structure of rapid-onset MDV-driven CD4+ lymphomas and responding CD8+ T cells[J]. Plos Pathogen,2011,7(5):e1001337.
[24] 任超. 巨型艾美耳球虫免疫激发宿主产生的CD8+ T细胞受体(TCR)谱系分析[D]. 北京:中国农业大学,2012.