关键词: 细胞培养, 猪囊尾蚴, 细胞系

Abstract: CC-97 cell lines of Cysticercus cellulosae with immunogenicity were established through culturing Cysticercus cellulosae cells in vitro. After culturing original generation cells for 30 days, primary monolayer cells were got. Then the monolayer cells were subcultured at 1:2 split ratio, and the next generation was subcultured every 7 days, totally, the cell lines were subcultured succesively for 24 generations. The cell line consisted of three cell types, the oval, the pear-shaped and the spherical cells under optical microscope. Among the cells, the oval cells were much more than others, while the number of pear-shaped cells and the number of spherical cells were nearly equal. Autoradiography method was used to detect cell division and ³H-TdR was used to analyze cell growth index, the two approaches demonstrated that the cell logarithmic proliferation phase was 7 days, the cell started to proliferate after culturing 12 hours and reached the proliferation peak at the 4th or 5th day during the culturing periods, and the proliferation rate reached 11 times, the amount of increment was 6.32×10⁹/L, the increment eventually reached the speed of 1:37 split ratio and the number of cells was 3.7×10¹⁰/L. The total length of cell cycle was about 32 hours, the cell line mainly consisted of diploid cells, the number of chromosomes was 10 pairs and each pair has 2 chromatids. There were 30 fractions of protein components, the analysis with esterase lsozymes showed a fragment with molecular weight around 32 kDa. Fluorescent antibody test showed that the cell line was homologous with Cysticercus cellulosae, the test results of tumorigenicity and exogenous contamination were negative. These results indicate that Cysticercus cellulosae cell line established in this research is a new biological strain of the homolog of Cysticercus cellulosae with uniform morphology, vigorous growth, stable hereditary, high immunogenicity, non-contamination and non-tumorigenicity.

Key words: cell culture, Cysticercus cellulosae, cell line