大口黑鲈北方亚种和佛罗里达亚种的同工酶分析

天津农学院学报 ›› 2012, Vol. 19 ›› Issue (3): 8-12.

大口黑鲈北方亚种和佛罗里达亚种的同工酶分析

张大莉¹, 杨蔷¹, 郝君¹, 刘斌², 李胜杰³, 董仕^{1,通讯作者}

1. 天津师范大学生命科学学院天津市细胞遗传与分子调控重点实验室,天津 300387;
2. 天津市天祥水产有限责任公司,天津 301500;
3. 中国水产科学研究院珠江水产研究所,广州 510380

收稿日期:2012-05-15 出版日期:2012-09-20 发布日期:2019-11-12
通讯作者: 董仕（1959-）,男,天津市人,研究员,博士,主要从事水产动物遗传育种学研究。E-mail：skyds@mail.tjnu.edu.cn。
作者简介:张大莉（1988-）,女,河南洛阳人,硕士在读,主要从事动物遗传学研究。E-mail：913801465@qq.com。
基金资助:
天津市农业科技成果转化与推广项目“大口黑鲈的引进与养殖示范”（201104120）; 科技部农业科技成果转化资金项目“大口黑鲈新品种及健康养殖技术转化与示范”（2012GB23260562）

Isozyme Analysis of Northern and Florida Subspecies of Largemouth Bass Micropterus salmoides

ZHANG Da-li¹, YANG Qiang¹, HAO Jun¹, LIU Bin², LI Sheng-jie³, DONG Shi^{1,Corresponding Author}

1. Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, College of Life Science, Tianjin Normal University, Tianjin 300387;
2. Tianjin Tianxiang Aquatic Company Limited, Tianjin 301500;
3. Pear River Fisheries Institute, Chinese Academy of Fishery Science, Guangzhou 510380

Received:2012-05-15 Online:2012-09-20 Published:2019-11-12

摘要/Abstract

摘要： 应用水平式淀粉凝胶电泳法,对2尾大口黑鲈的肌肉、肝脏、肾脏、心脏、眼、脑和鳃等7种组织的12种同工酶及蛋白质进行了检测筛选的预试验。实验认为,肌肉和肝脏的电泳带清晰、可以判别个体基因型的同工酶有7种,分别为AAT、GPI、IDH、LDH、MDH、ME和PGM。依据筛选结果,检测了50尾大口黑鲈北方亚种和40尾佛罗里达亚种肌肉和肝脏组织的7种同工酶,共检测出13个基因座位。除AAT-1*基因座位之外的12个基因座位上两个亚种群体内的个体间无变异,平均杂合度观察值Ho和平均杂合度预期值He均为0。IDH-1*和MDH-1*两个基因座位在两个亚种间有明显差异,佛罗里达亚种均具有*a基因,北方亚种均具有*b基因,可以此鉴定两个亚种。两亚种群体间的Nei遗传距为0.182 3。

关键词: 大口黑鲈, 同工酶, 遗传多样性

Abstract: Using horizontal starch gel electrophoresis method,the 12 isozymes of muscles,liver,kidney,heart,eye,brain and gill in two individuals of largemouth bass were examined. The seven isozumes of AAT,GPI,IDH,LDH,MDH,ME and PGM were useful for determining genotypes of largemouth bass, these seven isozymes of two subspecies of northern largemouth bass M. salmoides salmoides and Florida largemouth bass M. salmoides floridanus were examined. The 13 loci were observed, except AAT-1*, the polymorphic locus at 12 loci among individuals within subspecies in two subspecies were not found. The heterozygosities of Ho and He were zero. The genes of two subspecies at two loci of IDH-1* and MDH-1* were different, and this is useful for identifying the two subspecies. The Nei’s genetic distance between the two subspecies was 0.182 3.

Key words: largemouth bass（Micropterus salmoides）, isozyme, genetic diversity

中图分类号:

Q344.5

张大莉, 杨蔷, 郝君, 刘斌, 李胜杰, 董仕. 大口黑鲈北方亚种和佛罗里达亚种的同工酶分析[J]. 天津农学院学报, 2012, 19(3): 8-12.

ZHANG Da-li, YANG Qiang, HAO Jun, LIU Bin, LI Sheng-jie, DONG Shi. Isozyme Analysis of Northern and Florida Subspecies of Largemouth Bass Micropterus salmoides[J]. Journal of Tianjin Agricultural University, 2012, 19(3): 8-12.

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