氨氮对凡纳滨对虾稚虾的急性毒性作用

天津农学院学报 ›› 2012, Vol. 19 ›› Issue (3): 58-61.

氨氮对凡纳滨对虾稚虾的急性毒性作用

魏俊利¹, 孙金生^{1,2,通讯作者}, 李翔¹, 马文婷¹, 耿绪云¹, 徐赟霞¹, 李晶晶¹

1. 天津市水生动物疫病预防控制中心,天津 300221;
2. 天津师范大学细胞和分子调控重点实验室,天津 300387

收稿日期:2012-04-16 出版日期:2012-09-20 发布日期:2019-11-12
通讯作者: 孙金生（1963-）,男,天津市人,教授,学士,主要从事鱼类病害研究工作。联系电话：（022）8825078, E-mail：jssun1965@yahoo.com.cn。
作者简介:魏俊利（1975-）,男,天津市人,助理研究员,本科,主要从事鱼类病害研究。联系电话：（022）88252201,E-mail：wjl197503@yahoo.com.cn。
基金资助:
国家重点基础研究发展计划（973计划）项目“对虾白斑综合征防治的免疫学途径和关键技术原理”（2012CB114405）

Acute Toxic Effects of Ammonia Nitrogen on Litopenaeus vannamei

WEI Jun-li¹, SUN Jin-sheng^{1,2,Corresponding Author}, LI Xiang¹, MA Wen-ting¹, GENG Xu-yun¹, XU Yun-xia¹, LI Jing-jing¹

1. Tianjin Center for Control and Prevention of Aquatic Animal Infection Disease, Tianjin 300221, China;
2. Tianjin Key Laboratory of Cyto-genetical and Molecular Regulation, Tianjin Normal University, Tianjin 300387, China

Received:2012-04-16 Online:2012-09-20 Published:2019-11-12

摘要/Abstract

摘要： 实验研究了氨氮对体长3.2 cm、体质量0.356 g（n=10）与体长1.6 cm、体质量0.036 g（n=15）两种规格凡纳滨对虾（Litopenaeus vannamei）稚虾的急性毒性效应。血氨和碱性磷酸酶活性的测定结果显示,血氨随氨氮浓度的升高而升高,碱性磷酸酶（AKP）活性随氨氮的升高而下降。急性毒性实验结果表明,体长3.2 cm稚虾的游离态氨24 h LC₅₀为2.068 mg/L、48 h LC₅₀为1.654 mg/L、96 h LC₅₀为1.395 mg/L,生存安全浓度Sc为0.396 9 mg/L;体长1.6 cm稚虾的游离态氨24 h LC₅₀为1.577 mg/L、48 h LC₅₀为1.042 mg/L、96 h LC₅₀为1.012 mg/L,生存安全浓度Sc为0.206 5 mg/L。

关键词: 凡纳滨对虾稚虾, 碱性磷酸酶, 血氨, 游离态氨, 半数致死浓度, 生存安全浓度

Abstract: It is studied the acute toxic effects of ammonia nitrogen on two specifications of L. vannamei which mean body weights （MBW） were 0.356 g and 0.036 g, respectively. The concentrations of blood ammonia and activities of alkaline phosphatase（AKP） were analyzed. The results show that variation trend of concentrations of blood ammonia were consistent with the ammonia nitrogen, while the activities of alkaline phosphatase（AKP） were decreasing when the ammonia nitrogen raised. The median lethal concentrations （LC₅₀） of NH₃⁺on shrimp and the safe concentration（Sc） were also investigated. The LC₅₀of NH₃⁺on shrimp with MBW 0.356 g were 2.068 mg/L at 24 h, 1.654 mg/L at 48 h and 1.395 mg/L at 96 h, while the LC₅₀ of NH₃⁺on shrimp with MBW 0.036 g were 1.577 mg/L, 1.042 mg/L and 1.012 mg/L, respectively. The Sc was 0.396 9 mg/L and 0.206 5 mg/L to shrimp with MBW 0.356 g and 0.036 g, respectively. It is concluded that the changes of ammonia nitrogen have certain influence on L. vannamei, and the ammonia nitrogen was one of the important factors for the health and the survival of L. vannamei.

Key words: Litopenaeus vannamei, alkaline phosphatase, blood ammonia, free nitrogen, the median lethal concentration, the safe concentration

中图分类号:

S912

魏俊利, 孙金生, 李翔, 马文婷, 耿绪云, 徐赟霞, 李晶晶. 氨氮对凡纳滨对虾稚虾的急性毒性作用[J]. 天津农学院学报, 2012, 19(3): 58-61.

WEI Jun-li, SUN Jin-sheng, LI Xiang, MA Wen-ting, GENG Xu-yun, XU Yun-xia, LI Jing-jing. Acute Toxic Effects of Ammonia Nitrogen on Litopenaeus vannamei[J]. Journal of Tianjin Agricultural University, 2012, 19(3): 58-61.

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