基于咪唑基核壳式磁性金属有机框架的疫苗载体研究

doi:10.19640/j.cnki.jtau.2026.01.007

天津农学院学报 ›› 2026, Vol. 33 ›› Issue (1): 37-41.doi: 10.19640/j.cnki.jtau.2026.01.007

基于咪唑基核壳式磁性金属有机框架的疫苗载体研究

张志庚, 杨琳燕, 刁琦林, 刘欣华, 唐剑萌, 孙跃^通信作者

天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津 300392

收稿日期:2024-11-05 出版日期:2026-02-28 发布日期:2026-02-05
通讯作者: 孙跃（1986—）,女,助理实验师,学士,主要从事纳米药物载体研究。E-mail：443454387@qq.com。
作者简介:张志庚（2004—）,男,本科在读,主要从事纳米药物载体研究。E-mail：3191767611@qq.com。
基金资助:
国家自然科学基金（32202760）; 农业农村部智慧养殖重点实验室（省部共建）开放课题（2023-TJAULSBF-2103）; 天津市高等院校“兽医生物技术创新团队”资助项目（TD13-5091）; 天津市大学生创新创业训练计划项目（202410061156）

Studies on vaccines vectors based on imidazole based core-shell magnetic metal-organic framework

Zhang Zhigeng, Yang Linyan, Diao Qilin, Liu Xinhua, Tang Jianmeng, Sun Yue^{Corresponding Author}

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.

Received:2024-11-05 Online:2026-02-28 Published:2026-02-05

摘要/Abstract

摘要： 采用Zn金属盐或Co金属盐、2-甲基咪唑配体和羧基化磁性纳米微球通过一锅法制备核壳式金属有机框架。BSA作为模型疫苗,通过MOF材料自组装包封,在PBS缓冲液中释放。采用荧光分光光度法测定样品释放BSA的能力,从而探索咪唑基核壳式磁性金属有机框架材料在疫苗载体方面的潜力。红外光谱（IR）和X射线粉末衍射（XRD）对材料表征证实了γ- Fe₂O₃@SiO₂- NH₂-COOH/ZIF-8和γ- Fe₂O₃@SiO₂-NH₂-COOH/ZIF-67成功合成。体外释放BSA之后的材料的XRD测试数据表明材料发生结构坍塌。残余物特征衍射峰表明本研究制备的γ- Fe₂O₃@SiO₂-NH₂- COOH/ZIF-8和γ- Fe₂O₃@SiO₂-NH₂-COOH/ZIF-67作为疫苗载体,在体外释放后会自行瓦解。因此,制备的γ-Fe₂O₃@SiO₂-NH₂-COOH/ZIF-8和γ-Fe₂O₃@SiO₂-NH₂-COOH/ZIF-67可以为新型疫苗载体研发提供参考。

关键词: 磁性纳米微球, 磁性金属有机框架, BSA, 疫苗载体

Abstract: The core-shell metal-organic framework was synthesized by one-pot reaction with Zn metal salts, or Co metal salts, 2-methylimidazole ligands and Carboxylated magnetic nanoparticles in this research. BSA was encapsulated by MOF materials self-assembled and released in PBS buffer. The ability of the sample to release BSA was determined by fluorescence spectrophotometry, and the potential of imidazole based core-shell magnetic metal-organic framework materials in vaccine carriers was explored. By a variety of representational means, the synthesis of γ-Fe₂O₃@SiO₂-NH₂-COOH/ZIF-8 and γ-Fe₂O₃@SiO₂-NH₂-COOH/ZIF-67 were confirmed by infrared spectroscopy（IR）and X-ray diffraction（XRD）. The XRD measurements of used materials confirmed the materials used as vaccine vectors suffered structural collapse after the BSA released. The residue characteristic diffraction peaks indicated that the prepared γ-Fe₂O₃@SiO₂-NH₂-COOH/ZIF-8 and γ-Fe₂O₃@SiO₂-NH₂-COOH/ZIF-67 as vaccine vector would be disintegrated after release in vitro. Therefore, the prepared γ-Fe₂O₃@SiO₂-NH₂-COOH/ZIF-8 and γ-Fe₂O₃@SiO₂-NH₂-COOH/ZIF-67 can provide a reference for the development of new vaccine vectors.

Key words: magnetic nanoparticles, magnetic metal-organic framework, BSA, vaccine vectors

中图分类号:

O657

张志庚, 杨琳燕, 刁琦林, 刘欣华, 唐剑萌, 孙跃. 基于咪唑基核壳式磁性金属有机框架的疫苗载体研究[J]. 天津农学院学报, 2026, 33(1): 37-41.

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http://xuebao.tjau.edu.cn/CN/Y2026/V33/I1/37

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基于咪唑基核壳式磁性金属有机框架的疫苗载体研究

Studies on vaccines vectors based on imidazole based core-shell magnetic metal-organic framework

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