益生菌酸奶对小鼠免疫调节作用研究

doi:10.19640/j.cnki.jtau.2025.06.010

天津农学院学报 ›› 2025, Vol. 32 ›› Issue (6): 68-75.doi: 10.19640/j.cnki.jtau.2025.06.010

益生菌酸奶对小鼠免疫调节作用研究

许斐然¹, 运立媛^{1,3,4,通信作者}, 方曙光², 郭晓娟², 武岩峰², 张民^{1,3,4,通信作者}

1.天津农学院,食品科学与生物工程学院,天津 300392;
2.微康益生菌（苏州）股份有限公司,江苏苏州 215200;
3.农业农村部智慧养殖重点实验室,天津 300392;
4.天津市主要作物智能育种重点实验室,天津 300392

收稿日期:2024-11-28 发布日期:2025-12-26
通讯作者: 张民（1972—）,男,教授,博士,研究方向为食品化学与营养。E-mail：zhangmin@tjau.edu.cn;运立媛（1989—）,女,讲师,博士,研究方向为食品化学与营养。E-mail：yunly1120@163.com。
作者简介:许斐然（2000—）, 男, 硕士在读, 研究方向为食品化学与营养。E-mail：x1255894448@163.com。
基金资助:
国家重点研发计划（2022YFF1100201）; 天津农学院青年科技人才发展基金项目（2025QNKJ10）

Effect of probiotic yogurt on immune regulation in immunocompromised mice

Xu Feiran¹, Yun Liyuan^{1,3,4,Corresponding Author}, Fang Shuguang², Guo Xiaojuan², Wu Yanfeng², Zhang Min^{1,3,4,Corresponding Author}

1. School of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300392, China;
2. Wecare Probiotics Co., Ltd, Suzhou 215200, Jiangsu Province, China;
3. Key Laboratory of Smart Breeding, Ministry of Agriculture and Rural Affairs, Tianjin 300392, China;
4. Tianjin Key Laboratory of Intelligent Breeding of Major Crops, Tianjin 300392, China

Received:2024-11-28 Published:2025-12-26

摘要/Abstract

摘要： 本研究探讨了复合益生菌酸奶对环磷酰胺诱导免疫抑制小鼠免疫功能的影响。50只ICR小鼠被随机分为对照组（NC）、模型组（MC）、低剂量组（LPYC, 浓度：10⁹ CFU/250 g）、中剂量组（MPYC, 浓度：10¹⁰ CFU/250 g）和高剂量组（HPYC, 浓度：10¹¹ CFU/250 g）。通过测定小鼠体重、脏器指数、脾脏组织形态、免疫球蛋白及免疫细胞因子表达量, 探究益生菌酸奶对免疫低下小鼠免疫功能的影响。结果显示, MPYC组和HPYC组的小鼠体质量增长显著高于MC组（P<0.05）, 但低于NC组（P>0.05）。LPYC、MPYC和HPYC组小鼠的脾脏和胸腺指数均显著高于MC组（P<0.05）, 但体质量增长量显著低于NC组小鼠（P>0.05）。HE染色表明, 复合益生菌酸奶改善了小鼠脾脏组织的形态。ELISA显示, 与MC组相比, 复合益生菌酸奶显著提高了小鼠血清免疫球蛋白（IgG和IgA）的表达（P<0.05）。qRT-PCR结果表明, 复合益生菌酸奶显著提高了小鼠血清细胞因子TNF-α、IL-6和IL-12的表达（P<0.05）。综上所述, 复合益生菌酸奶对改善免疫低下小鼠的免疫功能具有显著效果。

关键词: 复合益生菌, 酸奶, 双歧杆菌, 免疫低下, 免疫活性

Abstract: This study explores the effects of a composite probiotic yogurt on the immune function of mice with immune suppression induced by cyclophosphamide. Fifty ICR mice were randomly divided into a control group（NC）, a model group （MC）, a low-dose group（LPYC, concentration: 10⁹ CFU/250 g）, a medium-dose group（MPYC, concentration: 10¹⁰ CFU/250 g）, and a high-dose group（HPYC, concentration: 10¹¹ CFU/250 g）. The study measured the mouse weight, organ index, morphology of the spleen tissue, expression levels of immunoglobulins and immune cell factors, to investigate the effects of probiotic yogurt on immune function. The results showed that the mouse weight growth of the MPYC group and HPYC group was significantly higher than that of the MC group（P<0.05）, but lower than that of the NC group（P>0.05）. The spleen and thymus index of the LPYC, MPYC, and HPYC groups were significantly higher than that of the MC group（P<0.05）, but significantly lower than the weight gain of the NC group mice（P>0.05）. HE staining showed that composite probiotic yogurt improved the morphology of the spleen tissue. ELISA showed that compared with the MC group, composite probiotic yogurt significantly increased the expression of immunoglobulin IgG and IgA in mouse serum（P<0.05）. qRT-PCR results showed that composite probiotic yogurt significantly increased the expression of immune cell factors TNF-α, IL-6, and IL-12 in mouse serum （P<0.05）. In conclusion, composite probiotic yogurt has a significant effect on improving the immune function of immune- suppressed mice.

Key words: compound probiotics, yogurt, Bifidobacterium, immunosuppression, immunoactivity

中图分类号:

R392-33

许斐然, 运立媛, 方曙光, 郭晓娟, 武岩峰, 张民. 益生菌酸奶对小鼠免疫调节作用研究[J]. 天津农学院学报, 2025, 32(6): 68-75.

Xu Feiran, Yun Liyuan, Fang Shuguang, Guo Xiaojuan, Wu Yanfeng, Zhang Min. Effect of probiotic yogurt on immune regulation in immunocompromised mice[J]. Journal of Tianjin Agricultural University, 2025, 32(6): 68-75.

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链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2025.06.010

http://xuebao.tjau.edu.cn/CN/Y2025/V32/I6/68

参考文献

[1] WANG X, ZHANG P, ZHANG X.Probiotics regulate gut microbiota:An effective method to improve immunity[J].Molecules, 2021, 26(19):6076.
[2] KAYISOGLU O, WEISS F, NIKLAS C, et al.Location-specific cell identity rather than exposure to GI microbiota defines many innate immune signalling cascades in the gut epithelium[J].Gut, 2021, 70(4):687-697.
[3] DE VRIES N L, VAN UNEN V, IJSSELSTEIJN M E, et al.High-dimensional cytometric analysis of colorectal cancer reveals novel mediators of antitumour immunity[J].Gut, 2020, 69(4):691-703.
[4] LI C, DUAN S, LI Y, et al.Polysaccharides in natural products that repair the damage to intestinal mucosa caused by cyclophosphamide and their mechanisms:A review[J].Carbohydrate Polymers, 2021, 261:117876.
[5] WU X, HUANG X, MA W, et al.Bioactive polysaccharides promote gut immunity via different ways[J].Food & Function, 2023, 14(3):1387-1400.
[6] DE VOS W M, TILG H, VAN HUL M, et al.Gut microbiome and health:Mechanistic insights[J].Gut, 2022, 71(5):1020-1032.
[7] FAN Y, STØVING R K, BERREIRA IBRAIM S, et al.The gut microbiota contributes to the pathogenesis of anorexia nervosa in humans and mice[J].Nature Microbiology, 2023, 8(5):787-802.
[8] MAGER L F, BURKHARD R, PETT N, et al.Microbiome-derived inosine modulates response to checkpoint inhibitor immunotherapy[J].Science, 2020, 369(6510):1481-1489.
[9] SONG W, WANG Y, LI G, et al.Modulating the gut microbiota is involved in the effect of low-molecular- weight Glycyrrhiza polysaccharide on immune function[J].Gut Microbes, 2023, 15(2):2276814.
[10] HE Q, ZHANG T, ZHANG W, et al.Administering Lactiplantibacillus fermentum F6 decreases intestinal Akkermansia muciniphila in a dextran sulfate sodium- induced rat colitis model[J].Food & Function, 2024, 15(11):5882-5894.
[11] CULLENDER T C, CHASSAING B, JANZON A, et al.Innate and adaptive immunity interact to quench microbiome flagellar motility in the gut[J].Cell Host & Microbe, 2013, 14(5):571-581.
[12] WAIDE M L, SCHMIDT N W.The gut microbiome, immunity, and Plasmodium severity[J].Current Opinion in Microbiology, 2020, 58:56-61.
[13] TANG C, DING R, SUN J, et al.The impacts of natural polysaccharides on intestinal microbiota and immune responses- A review[J].Food & Function, 2019, 10(5):2290-2312.
[14] LA FATA G, WEBER P, MOHAJERI M H.Probiotics and the gut immune system:Indirect regulation[J].Probiotics and Antimicrobial Proteins, 2018, 10(1):11-21.
[15] MASLOWSKI K M, MACKAY C R.Diet, gut microbiota and immune responses[J].Nature Immunology, 2010, 12(1):5-9.
[16] SANDERS M E, MERENSTEIN D J, REID G, et al.Probiotics and prebiotics in intestinal health and disease:From biology to the clinic[J].Nature Reviews Gastroenterology & Hepatology, 2019, 16(10):605-616.
[17] YUN L, WU T, LI Q, et al.Dietary supplementation with purified wheat germ glycoprotein improve immunostimulatory activity in cyclophosphamide induced Balb/c mice[J].International Journal of Biological Macromolecules, 2018, 118:1267-1275.
[18] ZHANG Z, MUHINDO E M, WANG S, et al.Structural characteristics and immunostimulatory activity of sea cucumber tendon polysaccharides in cyclophosphamide- induced Balb/c mice[J].Food & Function, 2022, 13(16):8627-8642.
[19] XIE H, FANG J, FARAG M A, et al.Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide- treated mice[J].Food Chemistry, 2022, 13:100235.
[20] TIAN B, WANG P, XU T, et al.Ameliorating effects of Hericium erinaceus polysaccharides on intestinal barrier injury in immunocompromised mice induced by cyclophosphamide[J].Food & Function, 2023, 14(6):2921-2932.
[21] 朱慧民, 李露露, 蔡远榛, 等.红豆杉多糖对免疫功能低下小鼠细胞免疫功能的影响[J].浙江医学, 2016, 38(20):1643-1645.
[22] YU Y, ZHU H, SHEN M, et al.Sulfation modification enhances the intestinal regulation of Cyclocarya paliurus polysaccharides in cyclophosphamide-treated mice via restoring intestinal mucosal barrier function and modulating gut microbiota[J].Food & Function, 2021, 12(24):12278-12290.
[23] MA W, SUN H, LIAN L, et al.Immunomodulatory effects of Lactiplantibacillus plantarum CCFM8661+stachyose on cyclophosphamide-induced immunosuppression mice[J].Frontiers in Immunology, 2025, 16:1513531.
[24] 李宗琪.益生菌BLa80和LA85的免疫调节作用研究[D].天津:天津科技大学, 2022.
[25] KADAOUI K A, CORTHÉSY B.Secretory IgA mediates bacterial translocation to dendritic cells in mouse peyer's patches with restriction to mucosal compartment[J].The Journal of Immunology, 2007, 179(11):7751-7757.
[26] AL AZZAZ J, RIEU A, AOUDIA N, et al.Probiotic Lactobacillus strains activities on autophagy and inflammatory response[J].Journal of Clinical Gastroenterology, 2016, 50(S2):S209.
[27] MALDONADO GALDEANO C, CAZORLA S I, LEMME DUMIT J M, et al.Beneficial effects of probiotic consumption on the immune system[J].Annals of Nutrition and Metabolism, 2019, 74(2):115-124.
[28] LIU J P, WANG J, ZHOU S X, et al.Ginger polysaccharides enhance intestinal immunity by modulating gut microbiota in cyclophosphamide-induced immunosuppressed mice[J].International Journal of Biological Macromolecules, 2022, 223:1308-1319.
[29] YOO J, GROER M, DUTRA S, et al.Gut microbiota and immune system interactions[J].Microorganisms, 2020, 8(10):1587.
[30] FAN L, QI Y, QU S, et al.B.adolescentis ameliorates chronic colitis by regulating Treg/Th2 response and gut microbiota remodeling[J].Gut Microbes, 2021, 13(1):1-17.

益生菌酸奶对小鼠免疫调节作用研究

Effect of probiotic yogurt on immune regulation in immunocompromised mice

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