天津农学院学报 ›› 2021, Vol. 28 ›› Issue (2): 18-24.doi: 10.19640/j.cnki.jtau.2021.02.004

• 研究与简报 • 上一篇    下一篇

野生山樱桃大小孢子发生和雌雄配子体发育过程的研究

杨楠1, 聂江力1,*, 辛微1, 裴毅1, 石福臣2   

  1. 1.天津农学院 园艺园林学院,天津 300392;
    2.南开大学 生命科学学院,天津 300384
  • 收稿日期:2020-05-06 出版日期:2021-06-30 发布日期:2021-07-26
  • 通讯作者: 聂江力(1972—),女,教授,博士,主要从事植物资源开发与利用。E-mail:njlnie@126.com。
  • 作者简介:杨楠(1996—),女,硕士在读,主要从事植物资源开发与利用。E-mail:ynbalabala@163.com。
  • 基金资助:
    天津市科技计划项目(18ZXZYNC00120; 16PTZSTG00020)

Studies on the development of microspores and male and female gametophytes in wild Cerasus serrulata

Yang Nan1, Nie Jiangli1,*, Xin Wei1, Pei Yi1, Shi Fuchen2   

  1. 1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China;
    2. College of life science, Nankai University, Tianjin 300384, China
  • Received:2020-05-06 Online:2021-06-30 Published:2021-07-26

摘要: 以天津蓟州区发现的野生山樱桃花芽和花蕾为试验材料,采用石蜡切片法对野生山樱桃大小孢子发生、雌雄配子体发育过程和雌雄性细胞发育的对应关系进行了研究。结果表明:野生山樱桃花药为蝶形,有4个药室,花药壁由内而外分别是绒毡层、中层、药室内壁和表皮,四分体为正四面体。大孢子发生过程中,孢原细胞分裂产生造孢细胞,进而分裂形成大孢子母细胞。大孢子母细胞减数分裂成线性排列的四分体,形成4个大孢子,最后只有1 个功能大孢子最终发育成胚囊。部分山樱桃子房中形成双胚珠,其中一个可以发育成成熟胚珠,另一个退化消失。山樱桃雄蕊发育早于雌蕊。

关键词: 山樱桃, 小孢子, 大孢子, 配子体发育

Abstract: In this experiment, Cerasus serrulata buds found in Jizhou district of Tianjin were used as experimental materials. The genesis of megaspore and microspore, the development of its gametophytes and the interrelationship between female and male cell development of Cerasus serrulata were studied by paraffin section. The results showed that Cerasus serrulata anthers were butterfly-shaped with 4 drug Chambers. The anther walls were tapetum, middle layer, chamber wall and epidermis from inside to outside. The tetrad was a regular tetrahedron. During megaspore generation, sporogenic cells divided to produce sporogenic cells, which then divided to form megaspore mother cells. Megaspore mother cells meiosis into linear arrangement of the tetrad formed four megaspores, but finally only one function megaspore eventually developed into the embryo sac. In some of the ovary, there weretwo ovules, one of which could mature into embryo sac while the other degenerated and disappeared. The stamens of Cerasus serrulata developed earlier than the pistil.

Key words: Cerasus serrulata, microspore, megaspore, gametophytic development

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