Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (4): 5-12.doi: 10.19640/j.cnki.jtau.2022.04.002

• Researches and Scientific Notes • Previous Articles     Next Articles

Study on extraction and fresh keeping effect of natural plant protection agent allicin

Chai Manqing1,2, Li Xiangxiang1, Wang Rui1, Zhang Jingjing1, Tian Lin1, Xu Xiaoping1,Corresponding Author   

  1. 1. College of Basic Science, Tianjin Agricultural University, Tianjin 300392, China;
    2. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2021-11-07 Online:2022-12-30 Published:2023-01-03

Abstract: The purpose of this experiment was to explore the most suitable conditions for the extraction of allicin and its application in the preservation of vegetables and fruits. The maximum absorption wavelength of allicin is 198 nm by UV full wavelength scanning. Taking the extraction rate of allicin as the index, the optimum extraction conditions of allicin were analyzed by orthogonal experiment under the action of five factors, namely, extraction temperature, extraction time, ethanol dosage, ultrasonic time and ultrasonic temperature. The results showed that the optimum extraction conditions of allicin with 85% ethanol were as follows: extraction temperature 50 ℃, extraction time 70 min, solid-liquid ratio 1∶5, ultrasonic tempercrture 30 ℃, ultrasonic time 50 min under power 600 W. The influencing factors of allicin extraction rate were extraction temperature>solid-liquid ratio>ultrasonic time>extraction time>ultrasonic temperature. Under the optimum conditions, the absorbance of allicin was 0.416 and the extraction rate of allicin was 4.60%. Through the fresh-keeping experiments of spinach, apple and banana, allicin has good fresh-keeping and antioxidant effects on vegetables and fruits.

Key words: allicin, organic solvent extraction, absorbance, fruit preservation, orthogonal experiment

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