Abstract: An immunosensor fabricated by immobilizing Brucella antibody onto the surface of gold nanoparticles-modified screen-printed carbon electrodes（GNPs-SPCEs）was proposed for the rapid detection of Brucella. The gold nanoparticle carbon electrode was used as the working electrode, and the Brucella antibody was modified on the surface of the working electrode to construct the electrochemical three electrode system. The reaction process was characterized by cyclic voltammetry（CV）. The change value of oxidation peak current（Δ IPA）was used as the current response signal, and PBS buffer was used as blank control. It was found that when the antigen of Brucella combined with antibody, the oxidation peak current decreased, while the current value of sterile PBS buffer almost did not change. When Escherichia coli and Staphylococcus aureus were used to replace Brucella, the response signal of interfering bacteria was similar to that of blank solution. Therefore, the immunosensor has good specificity for Brucella.

Key words: Brucella, gold nanoparticles, immunosensor, cyclic voltammetry, electrochemistry