红鳍东方鲀鱼皮胶原蛋白的提取及主要理化性质的研究

Journal of Tianjin Agricultural University ›› 2015, Vol. 22 ›› Issue (2): 6-10.

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Extraction and Physicochemical Characteristics of Collagen from Skin of Takifugurubripes

WAN Gang-tao¹, CHENG Min-jie¹, HUANG Ya-dong², WANG Qing-kui¹, WANG Guan¹, XING Ke-zhi^{1, *}

1. College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Haisheng Aquaculture Co. Ltd, Tianjin 300272, China

Received:2015-02-03 Published:2015-06-30

Abstract

Abstract: The acid-solubilized collagen（ASC）and pepsin-solubilized collagen （PSC）extracted from skin of Takifugurubripes were studied on its physicochemical properties, including the structure, purity, thermal denaturation temperature and the amino acid composition, etc. The results show that the ASC and PSC had high purity of 83.19 % and 85.00 %, respectively. The analysis of UV spectra, FTIR spectra and SDS-PAGE of ASC and PSC show that the two kinds of collagen were proved to be proteinⅠwith integrate triple-helix structure. And the thermal denaturation temperatures of ASC and PSC were 29.85 and 30.10 ℃, respectively.

Key words: Takifugurubripes, skin, collagen, physicochemical characteristics

CLC Number:

TS254.9

WAN Gang-tao, CHENG Min-jie, HUANG Ya-dong, WANG Qing-kui, WANG Guan, XING Ke-zhi. Extraction and Physicochemical Characteristics of Collagen from Skin of Takifugurubripes[J]. Journal of Tianjin Agricultural University, 2015, 22(2): 6-10.

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Extraction and Physicochemical Characteristics of Collagen from Skin of Takifugurubripes

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