葛沽萝卜松散型愈伤组织的诱导

doi:10.19640/j.cnki.jtau.2023.06.005

Journal of Tianjin Agricultural University ›› 2023, Vol. 30 ›› Issue (6): 22-26.doi: 10.19640/j.cnki.jtau.2023.06.005

• Researches and Scientific Notes • Previous Articles Next Articles

Friable callus induction of Gegu radish

Wang Zhaonan¹, Song Tieshan², Wang Nan¹, Nie Jiangli^{1, Corresponding Author}, Liu Yanjun¹, Pei Yi¹

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Jinnan District Siyuan Radish Cooperative, Tianjin 300354, China

Received:2022-11-12 Online:2023-12-31 Published:2024-02-05

Abstract

Abstract: In this study, leaf, petiole and hypocotyl of Gegu radish were used as explants to induce loose callus,to explore the effect of different explants and different hormone concentrations on the induction of loose callus. The results showed that different explants all could induce callus, but only cotyledon and petiole could induce loose callus, and the loose callus induced by cotyledon grew faster, and the callus induction rate was（26.88±1.44）%. Cotyledon was the most suitable explant for inducing loose callus. The optimal medium for induction of loose callus was MS+0.25 mg/L6-BA+2.0 mg/L 2,4-D. The induction rate of loose callus was（95.83±2.20）%, and the browning rate was only（0.83±0.83）%.

Key words: Gegu radish, cotyledon, friable callus

CLC Number:

S631.1

Wang Zhaonan, Song Tieshan, Wang Nan, Nie Jiangli, Liu Yanjun, Pei Yi. Friable callus induction of Gegu radish[J]. Journal of Tianjin Agricultural University, 2023, 30(6): 22-26.

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References

[1] 陈发波,刘毅,李文博,等. 胭脂萝卜初代培养及愈伤组织诱导分析[J]. 基因组学与应用生物学,2020,39(11):5157-5163.
[2] 胡伟,陈豫,伍洋. 不同激素浓度配比对萝卜愈伤组织形成的影响[J]. 江苏农业科学,2014,42(7):60-62.
[3] 王绍辉,高遐虹,程继鸿,等. 萝卜花药愈伤组织诱导及褐变因素初探[J]. 中国农学通报,2008,24(11):350-354.
[4] 朱献文,龚义勤,柳李旺,等. 萝卜花药愈伤组织诱导与分化影响因素的研究[J]. 生命科学研究,2007,11(1):90-94.
[5] 郭丽,索素敏,徐明辉. 组织培养中不同消毒处理对野生薄皮木种子萌发的影响[J]. 分子植物育种,2022,20(4):1325-1330.
[6] 邱诗春,张世平,冯山柴,等. 铁皮石斛种子快繁培养体系的优化[J]. 江苏农业科学,2021,49(3):58-61.
[7] 卞莹莹,颜彬,崔丽洁,等. 不结球白菜子叶离体培养与植株再生的研究[J]. 上海师范大学学报(自然科学版),2015,44(6):579-584.
[8] 党裳霓,高润梅,石晓东,等. 穿龙薯蓣种子离体再生体系构建及其组培苗解剖结构特征[J]. 西北植物学报,2021,41(4):585-594.
[9] 焦荟颖,裴毅,王晨曦,等. 黑穗醋栗‘布劳德’组培快繁体系的建立[J]. 分子植物育种,2020,18(20):6800-6807.
[10] 林珲,朱海生,陈敏氡,等. 花椰菜高频离体再生体系的构建[J]. 分子植物育种,2017,15(12):5060-5064.
[11] 万楚军. 西洋参子叶组织培养体系的建立[D]. 杨凌:西北农林科技大学,2022.
[12] 沈羊城,侯金艳,刘文博,等. 山桐子种子快速高效萌发研究[J]. 中国农学通报,2015,31(13):5-9.
[13] 乌云塔娜,包平安,乌日罕,等. 蒙古扁桃再生体系建立的研究[J]. 内蒙古农业大学学报(自然科学版),2021,42(6):23-29.
[14] 张宁,王义,刘心语,等. 外植体的种类及消毒方式对椰枣胚性愈伤组织诱导的影响[J]. 分子植物育种. 2022,20(10):3340-3346.
[15] 杨林军,谢彦云,李志锋,等. 分析中华常春藤中的化学成分[J]. 中草药,2016,47(4):566-572.
[16] 许丁帆,刘艳军,何远秦,等. 金银木松散型胚性愈伤组织的诱导[J]. 森林工程,2022,38(2):27-33.
[17] 夏凯丽,刘婷,杨静慧,等. 黑莓体细胞胚诱导中的细胞生理特性[J]. 中国南方果树,2018,47(1):114-118.
[18] 严小峰,刘艳军,黄俊轩,等. 冰灯玉露松散型胚性愈伤组织的诱导方法[J]. 天津农业科学,2017,23(7)21-24.

Friable callus induction of Gegu radish

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