基于γ-Fe2O3磁性纳米微球的过氧化物模拟酶研究

doi:10.19640/j.cnki.jtau.2022.01.010

天津农学院学报 ›› 2022, Vol. 29 ›› Issue (1): 47-51.doi: 10.19640/j.cnki.jtau.2022.01.010

基于γ-Fe₂O₃磁性纳米微球的过氧化物模拟酶研究

杨娇¹, 秦铭杉¹, 吕海军¹, 颜雪涛¹, 杨贵军², 杨琳燕^{1,通信作者}

1.天津农学院动物科学与动物医学学院,天津市农业动物繁育与健康养殖重点实验室,天津 300392;
2.天津市生物饲料添加剂企业重点实验室,天津 300383

收稿日期:2021-12-08 出版日期:2022-03-31 发布日期:2022-04-14
通讯作者: 杨琳燕（1982—）,女,讲师,博士,主要从事纳米药物载体研究。E-mail：y_linyan@163.com。
作者简介:杨娇（2000—）,女,本科在读,主要从事纳米药物载体研究。E-mail：2748090139@qq.com。
基金资助:
国家自然科学基金（31572492,31372482）; 天津农学院杰出科研业绩奖励资助项目（无编号）; 天津市教委科研计划项目（2017KJ190）; 天津市“兽医生物技术创新团队”资助项目（TD13-5091）; 广西师范大学省部共建药用资源化学与药物分子工程国家重点实验室资助课题（CMEMR2016-B12）; 天津市大学生创新创业训练计划项目（202010061066）

Studies on peroxidase mimics based on γ-F₂O₃ magnetic nanoparticles

Yang Jiao¹, Qin Mingshan¹, Lü Haijun¹, Yan Xuetao¹, Yang Guijun², Yang Linyan^{1,Corresponding Author}

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Key Laboratory of Biological Feed Additive Enterprises, Tianjin 300383, China

Received:2021-12-08 Online:2022-03-31 Published:2022-04-14

摘要/Abstract

摘要： 研究以γ-Fe₂O₃为基础的磁性纳米微球通过化学修饰是否可以具有过氧化物模拟酶的催化性质,通过制备SiO₂包被的γ-Fe₂O₃磁性纳米粒子,采用共价修饰方法对γ-Fe₂O₃@SiO₂磁性纳米粒子用葡萄糖氧化酶（GOD）修饰后得到γ-Fe₂O₃@SiO₂-NH₂-COOH-GOD磁性纳米微球,通过红外吸收光谱（IR）对γ-Fe₂O₃、γ-Fe₂O₃@SiO₂、γ-Fe₂O₃@SiO₂-NH₂、γ-Fe₂O₃@SiO₂-NH₂-COOH和γ-Fe₂O₃@SiO₂- NH₂-COOH-GOD磁性纳米微球进行结构确证。结果表明,磁性纳米微球的氨基化、羧基化及GOD修饰成功。利用荧光分光光度计测定后,证明γ-Fe₂O₃@SiO₂-NH₂-COOH-GOD具有过氧化物模拟酶的催化性质,可以催化H₂O₂和TMB之间的反应,葡萄糖的分解浓度过高酶也会出现淬灭现象。

关键词: γ-Fe₂O₃, GOD, 磁性纳米微球, 过氧化物模拟酶, 功能化修饰

Abstract: This article intends to study whether γ-Fe₂O₃-based magnetic nanoparticles can have the catalytic properties of peroxidase mimics by chemical modification. By preparing SiO₂-coated γ-Fe₂O₃ magnetic nanoparticles, and then adopting covalent modification methods, γ-Fe₂O₃@SiO₂ magnetic nanoparticles were modified with glucose oxidase (GOD), γ- Fe₂O₃@SiO₂-NH₂-COOH-GOD magnetic nanoparticles were obtained, and then γ-Fe₂O₃, γ-Fe₂O₃@SiO₂, γ-Fe₂O₃@SiO₂-NH₂, γ-Fe₂O₃@SiO₂-NH₂-COOH and γ-Fe₂O₃@SiO₂-NH₂-COOH-GOD magnetic nanoparticles were used for structural confirmation. The results showed that the amination, carboxylation and GOD-modification of the magnetic nanoparticles were successful. After measuring with a fluorescence spectrophotometer, γ-Fe₂O₃@SiO₂-NH₂-COOH-GOD was proved to have the catalytic properties of a peroxidase mimicking enzyme, which can catalyze the reaction between H₂O₂ and TMB. However, if the glucose decomposition concentration is too high, the enzyme quenching will also occur.

Key words: γ-Fe₂O₃, GOD, magnetic nanoparticles, peroxidase mimics, functional modification

中图分类号:

Q658.2

杨娇, 秦铭杉, 吕海军, 颜雪涛, 杨贵军, 杨琳燕. 基于γ-Fe₂O₃磁性纳米微球的过氧化物模拟酶研究[J]. 天津农学院学报, 2022, 29(1): 47-51.

Yang Jiao, Qin Mingshan, Lü Haijun, Yan Xuetao, Yang Guijun, Yang Linyan. Studies on peroxidase mimics based on γ-F₂O₃ magnetic nanoparticles[J]. Journal of Tianjin Agricultural University, 2022, 29(1): 47-51.

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http://xuebao.tjau.edu.cn/CN/Y2022/V29/I1/47

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基于γ-Fe₂O₃磁性纳米微球的过氧化物模拟酶研究

Studies on peroxidase mimics based on γ-F₂O₃ magnetic nanoparticles

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