探索DNA条形码对哈摇蚊类群部分属物种鉴定的有效性

doi:10.19640/j.cnki.jtau.2020.03.013

天津农学院学报 ›› 2020, Vol. 27 ›› Issue (3): 57-61.doi: 10.19640/j.cnki.jtau.2020.03.013

探索DNA条形码对哈摇蚊类群部分属物种鉴定的有效性

余海军, 黄鑫, 张钰莹, 周晶晶, 田召志, 陈佳林, 王茜^通信作者

天津农学院水产学院天津市水生生态及养殖重点实验室,天津 300384

收稿日期:2019-06-26 出版日期:2020-10-10 发布日期:2020-10-10
通讯作者: 王茜（1971-）,女,副教授,博士,主要从事水生动物及昆虫分子系统学的研究。E-mail：wqgt1999@163.com。
作者简介:余海军（1994-）,男,硕士在读,主要从事分子系统学研究。E-mail：yhj18322040463@163.com。
基金资助:
国家自然科学基金（31672264,31672324）

Exploring the utility of DNA barcoding in species identification of Harischia complex

YU Hai-jun, HUANG Xin, ZHANG Yu-ying, ZHOU Jing-jing, TIAN Zhao-zhi, CHEN Jia-lin, WANG Qian^{Corresponding Author}

Key Laboratory of Aquatic-Ecology and Aquaculture of Tianjin, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-06-26 Online:2020-10-10 Published:2020-10-10

摘要/Abstract

摘要： 采集中国部分地区哈摇蚊类群,并对其进行了DNA条形码的鉴别研究。研究结果显示：种间平均遗传距离大约是种内平均遗传距离的2.8倍,种内与种间存在明显的“DNA barcoding gap”。基于Automatic Barcode Gap Discovery（ABGD）法分析遗传距离与其分布频数的关系;邻接树（Neighbor joining tree,NJ tree）分析结果指出,87.5%的物种能区分开,并与形态学鉴定一致。本研究为探索DNA条形码在哈摇蚊类群中鉴别的有效性提供了基础数据,同时为填补中国摇蚊昆虫的DNA条形码数据提供丰富的材料。

关键词: 哈摇蚊类群, DNA条形码, 物种鉴定, 遗传距离

Abstract: This study collected Harischia complex species in regions of China, using DNA barcoding for species identification. The results showed: the mean interspecific genetic distance was 2.8 times larger than the mean intraspecific genetic distance, with existing distinct “DNA barcoding gap”. Based on Automatic Barcode Gap Discovery genetic distance was analyzed, and it was related with distributed frequency. The results of Neighbor joining tree analysis pointed that 87.5% of the species were distinguished, and it kept conformed to morphological identification. Our results produced basal data for exploring the utility of DNA barcoding in species identification of Harischia complex, and provided abundant material for filling up the Chinese DNA barcodes database of Chironomidae.

Key words: Harischia complex, DNA barcoding, species identification, genetic distance

中图分类号:

S931.1

余海军, 黄鑫, 张钰莹, 周晶晶, 田召志, 陈佳林, 王茜. 探索DNA条形码对哈摇蚊类群部分属物种鉴定的有效性[J]. 天津农学院学报, 2020, 27(3): 57-61.

YU Hai-jun, HUANG Xin, ZHANG Yu-ying, ZHOU Jing-jing, TIAN Zhao-zhi, CHEN Jia-lin, WANG Qian. Exploring the utility of DNA barcoding in species identification of Harischia complex[J]. Journal of Tianjin Agricultural University, 2020, 27(3): 57-61.

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链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2020.03.013

http://xuebao.tjau.edu.cn/CN/Y2020/V27/I3/57

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探索DNA条形码对哈摇蚊类群部分属物种鉴定的有效性

Exploring the utility of DNA barcoding in species identification of Harischia complex

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