不同时期花生茎叶中阿魏酸含量的提取及测定

doi:10.19640/j.cnki.jtau.2020.02.014

天津农学院学报 ›› 2020, Vol. 27 ›› Issue (2): 61-65.doi: 10.19640/j.cnki.jtau.2020.02.014

不同时期花生茎叶中阿魏酸含量的提取及测定

欧可可¹, 张平平^{1,2,通信作者}, 张丹丹¹, 赵晓彤¹

1.天津农学院食品科学与生物工程学院,天津 300384;
2.天津市农副产品深加工技术工程中心,天津 300384

收稿日期:2019-05-06 出版日期:2020-06-30 发布日期:2020-07-03
通讯作者: 张平平（1970-）,女,教授,博士,主要从事果蔬深加工研究。E-mail：zpp613@163.com。
作者简介:欧可可（1991-）,女,硕士在读,主要从事农副产品深加工及其利用研究。E-mail：15002276705@qq.com。
基金资助:
天津市科技计划项目（16YFNZNC00020）

Extraction and determination of ferulic acid in peanut stems and leaves at different stages

OU Ke-ke¹, ZHANG Ping-ping^{1,2,Corresponding Author}, ZHANG Dan-dan¹, ZHAO Xiao-tong¹

1. College of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Engineering and Technology Research Center of Agricultural Products Processing, Tianjin 300384, China

Received:2019-05-06 Online:2020-06-30 Published:2020-07-03

摘要/Abstract

摘要： 建立一种不同时期花生茎叶中阿魏酸含量的高效液相色谱（HPLC）检测方法并测定其含量。以水为溶剂,在单因素试验的基础上,通过正交试验优化花生茎叶中阿魏酸提取条件并优化检测条件。结果表明：HPLC最佳检测条件为色谱柱Venusil XBP C18,流动相乙腈∶0.3%磷酸水溶液为20∶80,流速1.0 mL/min,检测波长320 nm,在3.0~50.0 μg范围内,阿魏酸浓度与峰面积呈现良好的线性关系,平均回收率为100.4%,RSD=1.19%。此方法分析速度快、操作简便、结果的精密度与重现性好,可作为花生茎叶中阿魏酸含量的测定方法。阿魏酸最优提取条件：料液比为1∶100（g∶mL）、提取温度为95 ℃、提取时间为30 min;不同时期花生叶中阿魏酸含量均高于茎,其中初花期花生叶中阿魏酸含量是茎的2.1倍,成熟期花生叶中阿魏酸含量是茎的2.9倍。

关键词: 花生茎叶, 阿魏酸, 提取工艺, HPLC

Abstract: A high performance liquid chromatography（HPLC）method was established for the determination of ferulic acid in peanut stems and leaves at different stages. Based on the single factor experiments, orthogonal experiment was used to optimize extraction condition and the HPLC detection condition. The best HPLC condition was as follows: the analytical column was a Venusil XBP C18; The mobile phase consisted of acetonitrile-0.3% phosphoric acid aqueous solution（20∶80）; Flow rate was 1.0 mL/min, and detection wave length was 320 nm. In the range of 3.0~50.0 μg, there is a good linear relationship between ferulic acid concentration and the peak area. The average recovery of the method was 100.4% with a RSD of 1.19%（n=6）. This method has the advantages of fast analysis, simple operation, good precision and reproducibility, and is suitable for determination of ferulic acid content in peanut stems and leaves. The optimal extraction condition for ferulic acid was: ratio of material to liquid 1:100（g:mL）, extraction temperature 95 ℃, extraction time 30 min. The content of ferulic acid in peanut leaves was higher than that in stems at all stages. At initial flowering stage, the content of ferulic acid in peanut leaves was 2.1 times that of stems. At the ripening stage, the ferulic acid content was 2.9 times that of the stem.

Key words: peanut stems and leaves, ferulic acid, HPLC

中图分类号:

欧可可, 张平平, 张丹丹, 赵晓彤. 不同时期花生茎叶中阿魏酸含量的提取及测定[J]. 天津农学院学报, 2020, 27(2): 61-65.

OU Ke-ke, ZHANG Ping-ping, ZHANG Dan-dan, ZHAO Xiao-tong. Extraction and determination of ferulic acid in peanut stems and leaves at different stages[J]. Journal of Tianjin Agricultural University, 2020, 27(2): 61-65.

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参考文献

[1] 罗争辉,蔡百祥,梁益敏,等. 花生茎叶酚性成分研究[J]. 广西植物,2014,34(1):139-142.
[2] 王强. 花生加工品质学[M]. 北京:中国农业出版社,2013.
[3] 冯蓓蕾,陈淼,陆逸莹,等. 花生枝叶的研究现状[J]. 吉林中医药,2016,36(2):187-190.
[4] 张晓峰,庞传宇,王翘楚,等. 花生枝叶制剂的镇静催眠作用研究[J]. 中药药理与临床,2002,18(2):23-24.
[5] 郭福新,李如奎,许红,等. 落花生枝叶制剂对缺血性中风后失眠烦躁不安的疗效观察[C]//中国中西医结合学会. 中国中西医结合学会成立20周年纪念大会论文集. 北京:《中国学术期刊》电子杂志社,2001:168.
[6] 刘劲松,李莉,陈爱民,等. 花生茎叶不同提取部位对小鼠镇静催眠作用的初步探讨[J]. 中药材,2012,35(11):1833-1836.
[7] 李欣芮,陆永攀. 红皮、黑皮花生茎叶对小鼠镇静催眠作用的对比研究[J]. 西部中医药,2016,29(1):12-14.
[8] Zu X Y,Zhang Z Y,Xiong G Q,et al.Sedative effects of Arachis hypogaea L. stem and leaf extracts on sleep-deprived rats[J]. Experimental and Therapeutic Medicine,2013,6:601-605.
[9] 韦国锋,覃特营,何有成. 花生茎叶降压作用有效成分的[J]. 右江民族医学院学报,1991,13(2):11-13.
[10] 鉏晓艳,王玮琼,熊光权. 花生茎叶多糖提取工艺优化及抗氧化性研究[J]. 粮食与油脂,2017,30(7):99-102.
[11] 程雅芳,李全文,续颖,等. 花生茎中白藜芦醇的提取及体外抗氧化性的研究[J]. 食品工业科技,2011,32(7):144-146.
[12] Deng L,Shi A M,Liu H Z,et al.Identification of chemical ingredients of peanut stems and leaves extracts using UPLC-QTOF-MS coupled with novel informatics UNIFI platform[J]. Journal of Mass Spectrometry,2016,51(12):1157-1167.
[13] 邓磊. 花生茎叶提取物促睡眠活性物质鉴定及作用机制研究[D]. 北京:中国农业科学院,2017.
[14] 王强. 老香黄袋泡茶和老药桔袋泡茶制备工艺的研究[D]. 乌鲁木齐:新疆农业大学,2013.
[15] 寇莹莹,仝其根. 响应面法优化桂皮胶的提取工艺[J]. 食品工业科技,2018,39(2):156-162,167.
[16] 刘姗姗. 分心木袋泡茶制备工艺研究[D]. 乌鲁木齐:新疆农业大学,2015.

不同时期花生茎叶中阿魏酸含量的提取及测定

Extraction and determination of ferulic acid in peanut stems and leaves at different stages

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