一株高效解磷假单胞菌的分离鉴定及解磷特性

doi:10.19640/j.cnki.jtau.2019.04.010

天津农学院学报 ›› 2019, Vol. 26 ›› Issue (4): 49-53.doi: 10.19640/j.cnki.jtau.2019.04.010

一株高效解磷假单胞菌的分离鉴定及解磷特性

刘希旻¹, 杨海霞¹, 黄灵曦¹, 李晓雁², 权桂芝¹, 杨红澎¹, 黄海东^{1, 通信作者}

1. 天津农学院农学与资源环境学院,天津 300384;
2. 天津农学院食品科学与生物工程学院,天津 300384

收稿日期:2019-06-27 发布日期:2020-01-19
通讯作者: 黄海东（1972-）,男,教授,博士,研究方向：资源与应用微生物学。E-mail：hdhuang@tjau.edu.cn。
作者简介:刘希旻（1998-）,男,本科在读,研究方向：生物技术。E-mail：XmLiu19980119@163.com。
基金资助:
国家级大学生创新创业训练计划项目（201910061002）

Isolation and identification of a high-efficiency phosphate solubilizing Pseudomonas and its phosphate solubilizing characteristics

LIU Xi-min¹, YANG Hai-xia¹, HUANG Ling-xi¹, LI Xiao-yan², QUAN Gui-zhi¹, YANG Hong-peng¹, HUANG Hai-dong^{1, Corresponding Author}

1. College of Agronomy and Resources Environmentt, Tianjin Agricultural University, Tianjin 300384, China;
2. College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-06-27 Published:2020-01-19

摘要/Abstract

摘要： 磷是植物生长所必需的重要元素之一,土壤中的磷大都以难溶性磷的形式存在,难以被植物吸收利用。为提高盐碱地中磷的利用率,从天津滨海新区盐碱地土壤中筛选解磷细菌,在无机磷固体平板上分离得到一株能产生明显溶磷圈的菌株LQH-7,其溶磷指数为2.86,形态学、生理生化和基于16S rDNA基因序列的分子鉴定结果表明,LQH-7为维罗尼假单胞菌,与Pseudomonas veronii CIP 104663的同源性为99.65%。在初始pH为8.0,NaCl质量分数为0.5%的无机磷培养基中,30 ℃摇瓶发酵60 h,菌株LQH-7发酵液的pH为4.30,解磷量最高可达850.1 mg/L,说明该菌株在盐碱地土壤生物修复方面具有潜在的应用价值。

关键词: 解磷菌, 维罗尼假单胞菌, 菌种鉴定, 解磷能力

Abstract: ：Phosphorus is one of the important elements for plant growth. Plants cannot absorb enough phosphorus when a large amount of phosphorus in the soil exists in the form of insoluble state. To increase the utilization efficiency of phosphorus in saline-alkali, phosphate solubilizing bacteria were isolated from saline-alkali soil in Binhai New Area of Tianjin. Strain LQH-7, producing distinct soluble phosphorus circle with SPI 2.86, was isolated in inorganic phosphorus solid plate. Morphology, physiological-biochemical index and phylogenetic analysis based on 16S rDNA gene sequences showed that strain LQH-7 belonged to Pseudomonas veronii, exhibiting the highest sequence similarity with P. veronii CIP 104663（99.65%）. In inorganic phosphorus medium,under the fermentation conditions in which the initial pH was 8.0, NaCl content was 0.5%, temperature was 30 ℃ and fermentation time was 60 h, pH of strain LQH-7 fermentation broth reached 4.30 and the phosphate-solubilizing quantity reached its highest value, 850.1 mg/L, suggesting that LQH-7 is a potent candidate for bioremediation of saline-alkali soil.

Key words: phosphate solubilizing bacteria, Pseudomonas veronii, identification of bacteria, phosphate solubilizing ability

中图分类号:

S154.38

刘希旻, 杨海霞, 黄灵曦, 李晓雁, 权桂芝, 杨红澎, 黄海东. 一株高效解磷假单胞菌的分离鉴定及解磷特性[J]. 天津农学院学报, 2019, 26(4): 49-53.

LIU Xi-min, YANG Hai-xia, HUANG Ling-xi, LI Xiao-yan, QUAN Gui-zhi, YANG Hong-peng, HUANG Hai-dong. Isolation and identification of a high-efficiency phosphate solubilizing Pseudomonas and its phosphate solubilizing characteristics[J]. Journal of Tianjin Agricultural University, 2019, 26(4): 49-53.

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http://xuebao.tjau.edu.cn/CN/Y2019/V26/I4/49

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一株高效解磷假单胞菌的分离鉴定及解磷特性

Isolation and identification of a high-efficiency phosphate solubilizing Pseudomonas and its phosphate solubilizing characteristics

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