VBNC状态鸡白痢沙门氏菌快速诱导条件筛选

天津农学院学报 ›› 2013, Vol. 20 ›› Issue (3): 37-41.

VBNC状态鸡白痢沙门氏菌快速诱导条件筛选

胡烨¹, 马吉飞¹, 赵瑞利¹, 边艳², 杨久强³

1. 天津农学院动物科学系,天津 300384;
2. 天津市静海县畜牧水产业发展服务中心,天津 301600;
3. 天津市津南区畜牧水产业发展服务中心,天津 300350

收稿日期:2013-04-21 发布日期:2019-10-21
作者简介:胡烨（1984-）,女,吉林梨树人,实验师,硕士,主要从事动物微生物学研究。E-mail：star_0907@126.com。
基金资助:
天津农学院科技发展基金计划项目"VBNC沙门氏菌致病性研究"（2011N15）

Selection of VBNC State Rapid Induction Condition of Salmonella Pullorum

HU Ye¹, MA Ji-fei¹, ZHAO Rui-li¹, BIAN Yan², YANG Jiu-qiang³

1. Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China;
2. Jinghai Animal Husbandry and Aquaculture Development Service Center of Tianjin City, Tianjin 301600, China;
3. Jinnan Animal Husbandry and Aquaculture Development Service Center of Tianjin City, Tianjin 300350, China

Received:2013-04-21 Published:2019-10-21

摘要/Abstract

摘要： 为建立VBNC状态沙门氏菌实验室快速诱导条件,以4 ℃低温诱导为对照组,在此基础上,添加不同浓度Cu²⁺和冰醋酸作为快速诱导条件进行试验。结果表明,在4 ℃低温条件下,添加2~4 mM Cu²⁺对沙门氏菌进入VBNC状态有明显促进作用,与传统诱导条件相比,诱导时间平均提前15 d。对诱导进入VBNC状态的沙门氏菌进行16S rRNA鉴定,结果证明,所得菌为VBNC状态沙门氏菌。

关键词: 活的非可培养状态, 沙门氏菌, 16S rRNA, Cu²⁺

Abstract: In order to establish the rapid induction condition of VBNC state Salmonella pulorum, the induction at the low temperature of 4 ℃ was selected as the control group, and on this basis, the experiment was conducted through adding different concentrations of Cu²⁺ and glacial acetic acid as the rapid induction condition. The results show that adding 2~4 mM Cu²⁺ could obviously promote Salmonella into VBNC state at 4 ℃ low temperature, and compared with the traditional induction condition, the induction time could be advanced 15 d averagely. The Salmonella entering into the VBNC state was identified by 16S rRNA test, and the results proved that the stains were Salmonella of VBNC state.

Key words: viable but non-culturable state, Salmonella, 16S rRNA, Cu²⁺

中图分类号:

S852.614

胡烨, 马吉飞, 赵瑞利, 边艳, 杨久强. VBNC状态鸡白痢沙门氏菌快速诱导条件筛选[J]. 天津农学院学报, 2013, 20(3): 37-41.

HU Ye, MA Ji-fei, ZHAO Rui-li, BIAN Yan, YANG Jiu-qiang. Selection of VBNC State Rapid Induction Condition of Salmonella Pullorum[J]. Journal of Tianjin Agricultural University, 2013, 20(3): 37-41.

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