3种非特异性免疫相关基因在点带石斑鱼组织中的mRNA表达分析

天津农学院学报 ›› 2015, Vol. 22 ›› Issue (1): 1-5.

• 研究与简报 • 下一篇

3种非特异性免疫相关基因在点带石斑鱼组织中的mRNA表达分析

李晓萌, 戴伟, 方珍珍, 邢克智^*, 王庆奎, 陈成勋

天津农学院水产学院,天津 300384

收稿日期:2014-11-04 发布日期:2015-03-31
通讯作者: 国家自然科学基金（面上）项目“当归多糖对点带石斑鱼非特异性免疫和抗氧化机理的研究”（31270456）; “十二五”农村领域国家科技计划项目“黄渤海区鱼类工厂化健康养殖技术集成与示范”（2011BAD13B07）; 天津市科技支撑计划重点项目“中草药在水产健康养殖中的应用与开发”（13ZCZDNC00700）; 天津市科技特派员项目“当归提取物对石斑鱼免疫力的影响”（14JCTPJC00474）; 天津市高等学校创新团队基金项目“天津市‘现代水产健康养殖’创新团队基金项目”（TD12-5018）
作者简介:李晓萌（1989-）,女,天津市人,硕士在读,主要从事水产养殖方面的研究。E-mail：alice2069@hotmail.com。
基金资助:
邢克智（1956-）,男,天津市人,教授,学士,主要从事水产养殖方面的教学与研究工作。E-mail：kzxing6668@126.com。

mRNA Expression of Three Non-specific Immune Genes in Tissues of Epinephelus malabaricus

LI Xiao-meng, DAI Wei, FANG Zhen-zhen, XING Ke-zhi^*, WANG Qing-kui, CHEN Cheng-xun

College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2014-11-04 Published:2015-03-31

摘要/Abstract

摘要： 以β-肌动蛋白（β-actin）的表达量作为内标,应用半定量RT-PCR技术,对健康养殖条件下点带石斑鱼不同组织（头肾、心脏、肝脏、脾脏、胃、肠和鳃）中3种非特异性免疫相关基因：c型溶菌酶（CTL）、白细胞介素8（IL8）、toll样受体5（TLR5）的mRNA表达量进行了研究。结果显示,CTL、IL8、TLR5基因在点带石斑鱼的7种组织中均有表达,CTL基因表达量最高的组织为脾脏,IL8和TLR5基因在头肾中表达量最高;CTL、IL8、TLR5分别在肠、鳃和心脏中的表达量最低。

关键词: 点带石斑鱼, 半定量RT-PCR, 基因表达

Abstract: ：Using β-actin mRNA expression quantity as an internal reference, the mRNA expression differences of three non-specific immune genes, c-type lysozyme (CTL), interleukin 8 (IL8) and toll-like receptor 5 (TLR5), were analyzed in head kidney, heart, liver, spleen, stomach, intestine and gill of Epinephelus malabaricus with semi-quantitative RT-PCR technique. The results show that mRNA expressions of three genes were observed in all of the above tissues. The highest mRNA expression of CTL gene was detected in spleen while the highest mRNA expression for both IL8 and TLR5 genes was head kidney. The tissues with the lowest mRNA expressions for CTL, IL8 and TLR5 genes were intestine, gill and heart, respectively.

Key words: Epinephelus malabaricus, semi-quantitative RT-PCR, gene expression

中图分类号:

S965.334

李晓萌, 戴伟, 方珍珍, 邢克智, 王庆奎, 陈成勋. 3种非特异性免疫相关基因在点带石斑鱼组织中的mRNA表达分析[J]. 天津农学院学报, 2015, 22(1): 1-5.

LI Xiao-meng, DAI Wei, FANG Zhen-zhen, XING Ke-zhi, WANG Qing-kui, CHEN Cheng-xun. mRNA Expression of Three Non-specific Immune Genes in Tissues of Epinephelus malabaricus[J]. Journal of Tianjin Agricultural University, 2015, 22(1): 1-5.

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3种非特异性免疫相关基因在点带石斑鱼组织中的mRNA表达分析

mRNA Expression of Three Non-specific Immune Genes in Tissues of Epinephelus malabaricus

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