一株好氧反硝化细菌的分离鉴定及反硝化能力初测

天津农学院学报 ›› 2017, Vol. 24 ›› Issue (2): 44-48.

一株好氧反硝化细菌的分离鉴定及反硝化能力初测

战海^1,2, 王广军^{1,通信作者}, 陈成勋²

1. 中国水产科学研究院珠江水产研究所,广州 510380;
2. 天津农学院水产学院,天津 300384

收稿日期:2016-04-25 出版日期:2017-06-30 发布日期:2019-10-15
通讯作者: 王广军（1973–）,男,山东济宁人,研究员,硕士,主要从事水域环境调控研究。E-mail：wgj5810@163.com。
作者简介:战海（1989–）,男,山东烟台人,硕士在读,主要从事水域环境调控研究。E-mail：1033707611@qq.com。
基金资助:
广东省海洋渔业科技推广专项“内陆池塘复合净化技术示范与推广”（B20140C01）; “十二五”国家科技支撑计划项目“池塘优势生物群落调控管理技术研究”（2012BAD25B01）; 国家现代农业产业技术体系建设专项“草鱼养殖模式”（CARS-46-17）

Identification and Denitrification Ability of an Aerobic Denitrifier

ZHAN Hai^1,2, WANG Guang-jun^{1,Corresponding Author}, CHEN Cheng-xun²

1. Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
2. College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2016-04-25 Online:2017-06-30 Published:2019-10-15

摘要/Abstract

摘要： 采用BTB（溴甲基酚蓝）平板涂布分离法,从鳜鱼养殖池塘水体中分离出17株具有反硝化作用的细菌,通过初步筛选和反硝化能力的测定,挑选出一株具有较强反硝化能力的好氧反硝化细菌,命名为8F-3。菌株8F-3在24 h内将总氮从100.00 mg/L降至6.51 mg/L,去除率达到93.49%;将氨氮从50.000 mg/L降至1.966 mg/L,去除率为96.07%;将硝酸盐氮从50.00 mg/L降至3.51 mg/L,去除率为92.98%;将亚硝酸盐氮从0.096 mg/L降至0.071 mg/L,去除率为25.79%。该试验结果表明,菌株8F-3对氨氮和硝酸盐氮具有较强的去除能力,对亚硝酸盐氮也有一定去除作用,反硝化能力较强。经生理生化测试和16 S rRNA分子鉴定,初步鉴定该菌属于不动杆菌属（Acinetobacter）。

关键词: 好氧反硝化菌, 反硝化能力, 16S rRNA, 不动杆菌属

Abstract: 17 denitrification strains were isolated from mandarin fish pond water of Sanshui city using BTB （bromothymol blue） spread-plate technique, through the preliminary screening for denitrification strains and determination ability, one aerobic denitrifying bacteria which has strong determination, was named 8F-3. The results showed that the total content of nitrogen was reduced from 100.00 mg/L to 6.51 mg/L by 8F-3 strain in 24 hours and the rate of denitrification was 93.49%; Ammonia nitrogen was reduced from 50.000 mg/L to 1.966 mg/L, the removal rate was 96.07%, Nitrate nitrogen was reduced from 50.00 mg/L to 3.51 mg/L, the removal rate was 92.98%; Nitrite nitrogen was reduced from 0.096 mg/L to 0.071 mg/L, the removal rate was 25.79%. According to the blast results of 8F-3 strain 16S rRNA sequence （Bootstrap 1 000 times）and physiological test for 8F-3 using API method, the bacteria was finally identified as Acinetobacter sp.

Key words: aerobic denitrifier, denitrification ability, 16S rRNA, Acinetobacter

中图分类号:

S917.1

战海, 王广军, 陈成勋. 一株好氧反硝化细菌的分离鉴定及反硝化能力初测[J]. 天津农学院学报, 2017, 24(2): 44-48.

ZHAN Hai, WANG Guang-jun, CHEN Cheng-xun. Identification and Denitrification Ability of an Aerobic Denitrifier[J]. Journal of Tianjin Agricultural University, 2017, 24(2): 44-48.

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