黄连解毒汤对大鼠亚慢性毒性试验研究

天津农学院学报 ›› 2017, Vol. 24 ›› Issue (1): 23-27.

黄连解毒汤对大鼠亚慢性毒性试验研究

刘芳^2,3, 王立红^2,3, 董惠峰³, 赵晶晶^2,3, 张俊霞³, 刘鼎阔^{1,2,3,通信作者}

1. 中国农业大学动物医学学院,北京 100094;
2. 天津市饲用微生物制剂企业重点实验室,天津 300383;
3. 鼎正动物药业（天津）有限公司,天津 300383

收稿日期:2016-04-25 出版日期:2017-03-31 发布日期:2019-10-15
通讯作者: 刘鼎阔（1981-）,男,内蒙古赤峰人,畜牧师,硕士,主要从事中兽药新制剂开发。E-mail：liudingkuo1981@163.com。
作者简介:刘芳（1988-）,女,山东潍坊人,助理畜牧师,硕士,主要从事中兽药新制剂开发。E-mail：liuf1201@163.com。
基金资助:
天津市科技支撑计划项目“中草药在水产健康养殖中的应用与开发”（13ZCZDNC00700）

Subchronic Toxicity of Coptidis Toxin Resolving Decoction in Rats

LIU Fang^2,3, WANG Li-hong^2,3, DONG Hui-feng³, ZHAO Jing-jing^2,3, ZHANG Jun-xia³, LIU Ding-kuo^{1,2,3,Corresponding Author}

1. College of Veterinary Medicine at CAU , Beijing 100094, China;
2. Enterprise Key Laboratory of Feed Microbial Preparation, Tianjin 300383, China;
3. S&E animal pharmaceutical（Tianjin）Co, Ltd. , Tianjin 300383, China;

Received:2016-04-25 Online:2017-03-31 Published:2019-10-15

摘要/Abstract

摘要： 研究黄连解毒汤连续灌服情况下对于SD大鼠的亚慢性毒性作用,揭示其临床安全性。SD 大鼠120只,雌雄各半,随机分为对照组和黄连解毒汤 20、10、5 mL/kg 剂量组,每组 30 只,连续给药6周,以SD大鼠的体重、血液学、血液生化学、组织病理学检查作为检查指标,全面评价连续灌服对大鼠的毒性作用。连续给药42 d后,与对照组相比,黄连解毒汤试验组大鼠的血常规及各项血液生化检测指标结果相近（P>0.05）。组织病理学切片结果显示,黄连解毒汤20 mL/kg组大鼠肝脏和肾脏出现了轻微损伤,10 mL/kg和5 mL/kg 剂量组SD大鼠的的脾、肺、肝、肾、胃等组织亦均未发现异常的组织病理学变化。黄连解毒汤在10 mL/kg和5 mL/kg 剂量下连续灌服情况下对SD大鼠无不良影响。

关键词: 黄连解毒汤, 大鼠, 亚慢性毒性

Abstract: The subchronic toxicity of Coptidis Toxin Resolving Decoction in SD rats is researched by continuous perfusion in order to expose its clinical innocuity. 120 SD rats（males and females）were randomly devivided into control group and Coptidis Toxin Resolving Decoction in 3 different dosage groups（20, 10, and 5 mL/kg, 30 in each group）. The SD rats were administered with Coptidis Toxin Resolving Decoction by continuous perfusion for 6 weeks. The body weight, hematology, blood biochemistry and histopathology examination were measured for the overall toxicity assessment to rats. After 42 days, contrasted with the control group, the test group rats blood routine examination index and blood index had an approximately result（P>0.05）. The result of histopathological slices showed that the liver and kidney of Coptidis Toxin Resolving Decoction 20 mL/kg group rats appeared slight damage, while the spleen, lung, liver, kidney and stomach of Coptidis Toxin Resolving Decoction 10 mL/kg group and 5 mL/kg group does not find abnormal histopathologic change. Conclusion With a dose of 10 mL/kg and 5 mL/kg Coptidis Toxin Resolving Decoction continuous perfusion, there is not harmful effect on SD rats.

Key words: Coptidis Toxin Resolving Decoction, rat, subchronic toxicity

中图分类号:

S854.5

刘芳, 王立红, 董惠峰, 赵晶晶, 张俊霞, 刘鼎阔. 黄连解毒汤对大鼠亚慢性毒性试验研究[J]. 天津农学院学报, 2017, 24(1): 23-27.

LIU Fang, WANG Li-hong, DONG Hui-feng, ZHAO Jing-jing, ZHANG Jun-xia, LIU Ding-kuo. Subchronic Toxicity of Coptidis Toxin Resolving Decoction in Rats[J]. Journal of Tianjin Agricultural University, 2017, 24(1): 23-27.

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