牛蒡子中牛蒡苷的超声提取工艺优化研究

天津农学院学报 ›› 2016, Vol. 23 ›› Issue (4): 28-32.

牛蒡子中牛蒡苷的超声提取工艺优化研究

刘称,王英超^{1b,通信作者}, 金红, 马晓雨, 史雪艳

1. 天津农学院
a. 农学与资源环境学院,
b. 基础科学学院,天津 300384

收稿日期:2016-03-11 出版日期:2016-12-20 发布日期:2019-10-14
通讯作者: 王英超（1981-）,女,天津市人,实验师,硕士,主要从事生物化学实验教学与生物分离方面的研究。E-mail：shibiology@163.com。
作者简介:刘称（1993-）,女,天津市人,本科在读,主要从事生物分离与提取研究。E-mail：376147823@qq.com。
基金资助:
天津市大学生创新创业训练计划项目“牛蒡子中牛蒡子苷的提取方法比较、优化及其利用”（20151006105）; 天津市科技支撑项目“枣活性成分功效研究及产品开发”（11ZCKFNC01600）

Study on the Extraction Conditions of Arctiin in Ultrasonic Method

LIU Chen, WANG Ying-chao^{1b,Corresponding Author} JIN Hong, MA Xiao-yu, SHI Xue-yan

1. Tianjin Agricultural University,
a. College of Agronomy and Resource Environment,
b. College of Basic Science,Tianjin 300384,China

Received:2016-03-11 Online:2016-12-20 Published:2019-10-14

摘要/Abstract

摘要： 利用超声法对牛蒡苷最佳提取条件进行优化研究。考察超声功率、超声时间、超声温度、料液比等因素对牛蒡苷提取率的影响,并采用正交试验优化提取条件。结果表明：粒径为100目、提取溶剂为95%乙醇、预浸泡时间2 h、料液比1∶150（g∶mL）、超声时间45 min、超声功率 114 W、超声温度70 ℃为最佳提取条件。在最佳提取条件下,进行了3组平行验证试验,牛蒡苷的平均提取率为12.69%。与其他超声提取工艺相比,牛蒡苷提取率至少提高1.89%。

关键词: 牛蒡子, 牛蒡苷, 含量测定

Abstract: The optimal extraction condition of arctiin was studied by ultrasonic method. The newly fresh seed of the Arctium lappa was used to investigate the effects of the ultrasonic power, ultrasonic time, ultrasonic temperature, solid-liquid ratio and other factors on the extraction rate of arctiin. Orthogonal experiment was performed to optimize the extraction conditions. The results showed that the crush size was 100 mesh, extraction solvent was 95% ethanol, pre-soaking time was 2 h, material liquid ratio was 1∶150 (g∶mL), ultrasonic time was 45 min, ultrasonic power was 114 W, ultrasonic temperature was 70 ℃ as the optimum extraction conditions. The three parallel tests were carried out under the optimum extraction conditions, the average extraction rate obtained for arctiin was 12.69% . Compared with other ultrasonic extraction process, arctiin yield was increased at least 1.89%.

Key words: the seeds of the Arctium lappa, arctiin, content determination

中图分类号:

R284.2

刘称,王英超, 金红, 马晓雨, 史雪艳. 牛蒡子中牛蒡苷的超声提取工艺优化研究[J]. 天津农学院学报, 2016, 23(4): 28-32.

LIU Chen, WANG Ying-chao JIN Hong, MA Xiao-yu, SHI Xue-yan. Study on the Extraction Conditions of Arctiin in Ultrasonic Method[J]. Journal of Tianjin Agricultural University, 2016, 23(4): 28-32.

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http://xuebao.tjau.edu.cn/CN/Y2016/V23/I4/28

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