革胡子鲶感染维氏气单胞菌脾脏c型溶菌酶基因表达及序列的初步分析

doi:10.19640/j.cnki.jtau.2026.03.004

天津农学院学报 ›› 2026, Vol. 33 ›› Issue (3): 19-24.doi: 10.19640/j.cnki.jtau.2026.03.004

革胡子鲶感染维氏气单胞菌脾脏c型溶菌酶基因表达及序列的初步分析

蓝一¹, 张纯玮¹, 王晓梅^1,2, 王茜^{1,2,通信作者}

1.天津农学院水产学院,天津 300392;
2.天津市水生生态及养殖重点实验室,天津 300392

收稿日期:2024-03-03 出版日期:2026-06-30 发布日期:2026-06-30
通讯作者: 王茜（1971—）,女,教授,博士,主要从事水产增养殖、水产遗传与育种方面的研究。E-mail：wqgt1999@163.com。
作者简介:蓝一（1999—）,男,硕士在读,主要从事水产增养殖、水产遗传与育种方面的研究。E-mail：layi3018@163.com。
基金资助:
国家自然科学基金（31672264）; 天津市淡水养殖产业体系创新团队建设项目（ITTFRSA2021000）

Preliminary analysis of c-type lysozyme gene expression and sequence in the spleen of Clarias gariepinus infected with Aeromonas veronii

Lan Yi¹, Zhang Chunwei¹, Wang Xiaomei^1,2, Wang Qian^{1,2,Corresponding Author}

1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. Key Laboratory of Aquatic-ecology and Aquaculture of Tianjin, Tianjin 300392, China

Received:2024-03-03 Online:2026-06-30 Published:2026-06-30

摘要/Abstract

摘要： 本试验应用维氏气单胞菌（Aeromonas veronii）人工感染革胡子鲶（Clarias gariepinus）,依据健康革胡子鲶脾脏与感染后3 h、24 h和48 h革胡子鲶脾脏的转录组数据信息,分析其c型溶菌酶Unigene序列和c型溶菌酶基因的表达水平。结果表明：c型溶菌酶Unigene序列长为742 bp,其中CDS区位点为99~539,共441 bp;在257和502位点发生了G→A和C→T碱基转换,在86位点发生多个碱基插入或缺失,故导致有两种基因型GGTCTAA和G------存在。攻菌后48 h内革胡子鲶脾脏c型溶菌酶表达量持续上升,在48 h时达到最大值,且显著高于细菌感染后3 h、24 h以及健康革胡子鲶脾脏c型溶菌酶的表达量。本文对革胡子鲶c型溶菌酶基因CDS序列和NCBI上查询的其他8种鱼c型溶菌酶基因CDS序列进行聚类分析。结果表明革胡子鲶与蟾胡鲶（Clarias batrachus）c型溶菌酶基因CDS序列相似性最高,为95.24%。本研究为探讨革胡子鲶脾脏中c型溶菌酶表达和序列特征提供了初步见解,为进一步深入研究该基因在免疫系统中的功能奠定了基础。

关键词: 革胡子鲶, 转录组分析, c型溶菌酶, 基因表达

Abstract: This experiment involved the artificial infection of Clarias gariepinus with Aeromonas veronii. Based on the transcriptome data of the spleen from healthy C. gariepinus and those infected for 3, 24, and 48 hours, the expression levels of the c-type lysozyme Unigene sequence and c-type lysozyme gene were analyzed. The results revealed that the c-type lysozyme Unigene sequence was 742 bp long, with the coding sequence（CDS）region spanning from position 99 to 539, totaling 441 bp. At positions 257 and 502, there were transitions of G→A and C→T bases, while at position 86, there were multiple base insertions or deletions, resulting in two genotypes: GGTCTAA and G------. The expression level of c-type lysozyme in the spleen of C. gariepinus continued to increase within 48 hours post-infection, reaching its peak at 48 hours, significantly higher than the expression levels at 3 and 24 hours post-bacterial infection and that of healthy C. gariepinus spleen. Clustering analysis was performed on the CDS sequences of the c-type lysozyme gene in C. gariepinus and eight other fish species retrieved from NCBI. The results indicated that the similarity between the c-type lysozyme gene CDS sequences of C. gariepinus and Clarias batrachus was the highest, at 95.24%. This study provides preliminary insights into the expression and sequence characterization of c-type lysozyme in the spleen of C. gariepinus, and lays foundation for further in-depth studies on the function of this gene in the immune system.

Key words: Clarias gariepinus, transcriptome analysis, c-type lysozyme, gene expression

中图分类号:

S943

蓝一, 张纯玮, 王晓梅, 王茜. 革胡子鲶感染维氏气单胞菌脾脏c型溶菌酶基因表达及序列的初步分析[J]. 天津农学院学报, 2026, 33(3): 19-24.

Lan Yi, Zhang Chunwei, Wang Xiaomei, Wang Qian. Preliminary analysis of c-type lysozyme gene expression and sequence in the spleen of Clarias gariepinus infected with Aeromonas veronii[J]. Journal of Tianjin Agricultural University, 2026, 33(3): 19-24.

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http://xuebao.tjau.edu.cn/CN/Y2026/V33/I3/19

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革胡子鲶感染维氏气单胞菌脾脏c型溶菌酶基因表达及序列的初步分析

Preliminary analysis of c-type lysozyme gene expression and sequence in the spleen of Clarias gariepinus infected with Aeromonas veronii

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