天津沿海牡蛎的形态学观察及分子鉴定

doi:10.19640/j.cnki.jtau.2023.01.010

天津农学院学报 ›› 2023, Vol. 30 ›› Issue (1): 46-50.doi: 10.19640/j.cnki.jtau.2023.01.010

天津沿海牡蛎的形态学观察及分子鉴定

于帅, 刘利华, 李永仁, 郭永军, 陈丽梅^通信作者

天津农学院水产学院天津市水产生态及养殖重点实验室,天津 300392

收稿日期:2021-07-10 出版日期:2023-03-31 发布日期:2023-04-21
通讯作者: 陈丽梅（1981—）,女,副教授,博士,主要从事水产养殖与遗传育种方面研究。E-mail：chenlimeicc@163.com。
作者简介:于帅（1997—）,女,硕士在读,主要从事水产养殖方面研究。E-mail：yyushuai99@163.com。
基金资助:
天津市海水养殖现代农业产业技术体系项目（ITTMRS2021003）; 国家现代农业产业技术体系项目（CARS-48）

Morphological observation and molecular identification of oyster in Tianjin coastal area

Yu Shuai, Liu Lihua, Li Yongren, Guo Yongjun, Chen limei^{Corresponding Author}

Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2021-07-10 Online:2023-03-31 Published:2023-04-21

摘要/Abstract

摘要： 为了明确天津沿海的牡蛎种类,在独流减河口、北疆电厂防波堤和东疆港3个采样点共采集了127个野生牡蛎,首先对牡蛎样本进行形态学指标测量,然后通过细胞色素氧化酶Ⅰ（cytochrome oxidase I,COI）基因序列分析,对牡蛎种类进行进一步鉴定。结果表明,16个COI基因序列共得到9个单倍型,单倍型多样性（h）为0.767,核苷酸多样性（π）为0.001 73;聚类分析结果表明,本研究得到的牡蛎序列首先和长牡蛎（Crassostrea gigas）聚为一支,然后和福建牡蛎（Crassostrea angulata）聚为一支。综合形态学观察和分子鉴定的结果,确定天津沿海3个地点采集的牡蛎样本均属于长牡蛎,说明天津海域的牡蛎种类组成较为单一。此次研究并未发现前期报道中的猫爪牡蛎（Talonostrea talonata）及近江牡蛎（Crassostrea ariakensis）,需要进一步增加采样点以得到更全面的结论。研究结果对了解天津野生牡蛎资源状况、保护物种多样性具有重要的意义。

关键词: 天津沿海, 牡蛎, 形态学指标, 细胞色素氧化酶Ⅰ, 分子鉴定

Abstract: To understand the species of oysters in the coastal area of Tianjin, a total of 127 wild oysters were collected from three sampling sites, including the Duliujianhe river estuary, Beijiang Power Plant Breakwater and Dongjiang Port. First, morphological indexes were measured, and then cytochrome oxidase I（COI）gene sequence was analyzed. The oyster species were further identified. The results showed that 9 haplotypes were obtained from 16 COI gene sequences. The Haplotype diversity（h）and Nucleotide diversity（π）were 0.767 and 0.001 73 respectively. Cluster analysis results showed that the oyster sequences obtained in this study first clustered into a branch with Crassostrea gigas, and then clustered into a branch with Crassostrea angulata. Based on the results of morphological observation and molecular identification, the oyster samples collected from the three locations along the coast of Tianjin were all Crassostrea gigas, indicating that the composition of oyster species in Tianjin sea area was relatively simple. The Talonostrea talonata and Crassostrea ariakensis as previously reported were not found in this study, and further sampling sites are needed to obtain more comprehensive conclusions. The results of this study are of great significance for understanding the status of existing oyster resources and protecting species diversity in Tianjin.

Key words: Tianjin coast, oyster species, morphology index, cytochrome oxidase I, molecular identification

中图分类号:

S932.6

于帅, 刘利华, 李永仁, 郭永军, 陈丽梅. 天津沿海牡蛎的形态学观察及分子鉴定[J]. 天津农学院学报, 2023, 30(1): 46-50.

Yu Shuai, Liu Lihua, Li Yongren, Guo Yongjun, Chen limei. Morphological observation and molecular identification of oyster in Tianjin coastal area[J]. Journal of Tianjin Agricultural University, 2023, 30(1): 46-50.

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天津沿海牡蛎的形态学观察及分子鉴定

Morphological observation and molecular identification of oyster in Tianjin coastal area

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