对虾肠道微囊藻毒素厌氧降解菌的分离鉴定及其降解性能比较分析

doi:10.19640/j.cnki.jtau.2025.05.009

Journal of Tianjin Agricultural University ›› 2025, Vol. 32 ›› Issue (5): 57-63.doi: 10.19640/j.cnki.jtau.2025.05.009

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Isolation, identification and comparative analysis of the degradation performance of anaerobic microcystins-degrading bacteria from shrimp intestine

Huang Jingxian, Wu Xian, Wang Xiaolin, Chen Yiwen, Dai Wei^{Corresponding Author}

College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2025-05-19 Published:2025-11-12

Abstract

Abstract: To mitigate the toxic effects of microcystin（MCs）pollution in aquaculture waters on shrimp, this study isolated five anaerobic MCs-degrading bacterial strains from the intestines of shrimp cultured in Microcystis-blooming pond through selective enrichment culture. The strains were identified using morphological observation, physiological and biochemical characterization, and 16S rRNA gene sequence analysis, followed by evaluation of their degradation performance. Results showed that five MCs-degrading strains were identified as Shewanella algae, Serratia marcescens, Citrobacter amalonaticus, Exiguobacterium mexicanum, and Bacillus flexus. The MCs-degrading efficiency of the five strains followed the descending order: B. flexus>E. mexicanum>S. marcescens>C. amalonaticus>S. algae. Under an initial MCs concentration of 390.17 μg/L,B. flexus and E. mexicanum （4.5×10⁸ CFU/mL） achieved complete MCs degradation within 48 h, while S. marcescens（at the same cell density）required 72 h for complete degradation. Among the five bacteria strains, B. flexus and S. marcescens show potential as feed additives for MCs pollution control in shrimp aquaculture.

Key words: shrimp, microcystins, anaerobic degradation, bacterial strain, isolation, identification

CLC Number:

S949

Huang Jingxian, Wu Xian, Wang Xiaolin, Chen Yiwen, Dai Wei. Isolation, identification and comparative analysis of the degradation performance of anaerobic microcystins-degrading bacteria from shrimp intestine[J]. Journal of Tianjin Agricultural University, 2025, 32(5): 57-63.

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Isolation, identification and comparative analysis of the degradation performance of anaerobic microcystins-degrading bacteria from shrimp intestine

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